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Clinical Chemistry 8: 151-157, 1962;
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Clinical Chemistry, Vol 8, 151-157, Copyright © 1962 by the American Association for Clinical Chemistry

New Method for the Determination of Whole-Blood Iron and Hemoglobin

Harold V. Connerty 1 and Anglis R. Briggs 1

1 Department of Pathology, Veterans Administration Hospital, Wilmington, Dela.

A rapid, simple, highly accurate procedure for the determination of hemoglobin iron is described. The use of a sodium hypochlorite solution achieves a smoother, faster, cleaner digestion of hemoglobin and release of the iron than the use of concentrated sulfuric acid and persulfate.

The accuracy of the method is established by recovery experiments showing that a linear relation exists between the volume of a given hemoglobin solution analyzed and the total amount of iron released, as well as by the demonstration that a constant fixed ratio exists between hemoglobin iron and hemoglobin (as cyanmethemoglobin).

The new method is easier to perform than the time-honored procedure of Wong.

Submitted on May 3, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.