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Clinical Chemistry 8: 266-269, 1962;
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Clinical Chemistry, Vol 8, 266-269, Copyright © 1962 by the American Association for Clinical Chemistry

Paper Chromatographic Estimation of Phenylalanine and Tyrosine Using Finger-tip Blood

Its Application to Phenylketonuria

W. J. Culley 1, E. T. Mertz 1, M. W. Luce 1, J. M. Calandro 1, and D. H. Jolly 1

1 Mental Retardation Research Laboratory, Muscatatuck State School, Butlerville, Ind., and the Biochemistry Department, Purdue University, Lafayette, Ind.

A paper chromatographic method for the determination of serum phenylalanine and tyrosine has been developed that requires only 0.12 ml. of serum, no deproteinization, and no expensive equipment. This method has been applied to the detection of phenylketonuria and to check serum levels of phenylketonurics on low-phenylalanine diets.

Submitted on July 11, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.