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Clinical Chemistry 8: 284-288, 1962;
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Clinical Chemistry, Vol 8, 284-288, Copyright © 1962 by the American Association for Clinical Chemistry

A Method for the Fractionation and Quantitative Determination of the 17-Oxosteroids of Urine

David J. Watson 1 and Paige K. Besch 1

1 Department of Obsterics and Gynecology, Ohio State Medical School, Columbus, O.

A method for the quantitative fractionation and estimation of urinary 17-oxosteroids is presented. The method utilizes paper chromatographic technics for separation of the steroids, which are quantitated with the Zimmerman reaction using m-dinitrobenzene. Details of the chromatographic procedure are given, with a comparison of this with other methods in use.

Submitted on July 21, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.