Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 8: 302-309, 1962;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bowman, R. E.
Right arrow Articles by Wolf, R. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bowman, R. E.
Right arrow Articles by Wolf, R. C.

Clinical Chemistry, Vol 8, 302-309, Copyright © 1962 by the American Association for Clinical Chemistry

A Rapid and Specific Ultramicro Method for Total Serum Cholesterol

Robert E. Bowman 1 and Richard C. Wolf 1

1 Primate Laboratory and Department of Physiology, University of Wisconsin, Madison 6, Wise.

A modification of the Zak cholesterol reaction is described in which ethanol is substituted for glacial acetic acid. This modification avoids the hazard of purifying glacial acetic acid and also increases the specificity of the reaction by eliminating interference from bromide or iodide salts. The ethanolic reaction has the absorbance spectrum, sensitivity, and stability of the usual acetic acid reaction.

A rapid ultramicro method has been developed based on this ethanolic reaction. A 0.01- to 0.05-ml. sample of serum is extracted with 2.5 ml. of ethanol, and a 2.0-ml. aliquot of this extract is reacted directly with 2.0 ml. of a Zak color reagent. Cholesterol concentrations as determined by this procedure and by the Sperry-Webb method showed satisfactory agreement for monkey and human sera, including normal, hemolyzed, high cholesterol, and severely lipemic or jaundiced sera.

. Submitted on March 6, 1961




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
R. A. Canuto, G. Muzio, M. Maggiora, A. Trombetta, G. Martinasso, R. Autelli, P. Costelli, G. Bonelli, and F. M. Baccino
Apoptosis induced by clofibrate in Yoshida AH-130 hepatoma cells: role of HMG-CoA reductase
J. Lipid Res., January 1, 2003; 44(1): 56 - 64.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
P. Costelli, L. Tessitore, B. Batetta, M. F. Mulas, O. Spano, P. Pani, F. M. Baccino, and S. Dessì
Alterations of Lipid and Cholesterol Metabolism in Cachectic Tumor-Bearing Rats Are Prevented by Insulin
J. Nutr., March 1, 1999; 129(3): 700 - 706.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
P. Vijayagopal and D. L. Glancy
Macrophages Stimulate Cholesteryl Ester Accumulation in Cocultured Smooth Muscle Cells Incubated With Lipoprotein-Proteoglycan Complex
Arterioscler. Thromb. Vasc. Biol., September 1, 1996; 16(9): 1112 - 1121.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. A. Araujo, E. L. Romano, B. E. Brito, V. Parthe, M. Romano, M. Bracho, R. F. Montano, and J. Cardier
Iron Overload Augments the Development of Atherosclerotic Lesions in Rabbits
Arterioscler. Thromb. Vasc. Biol., August 1, 1995; 15(8): 1172 - 1180.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1962 by the American Association for Clinical Chemistry.