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Clinical Chemistry 8: 343-350, 1962;
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Clinical Chemistry, Vol 8, 343-350, Copyright © 1962 by the American Association for Clinical Chemistry

Serum Glutamic-Oxaloacetic Transaminase Activity

A New Modification and an Analytical Assessment of Current Assay Technics

Elias Amador 1 and Warren E. C. Wacker 1

1 Biophysics Research Laboratory, Division of Medical Biology of the Department of Medicine, Harvard Medical School and Peter Bent Brigham Hospital, Boston, Mass.

The spectrophotometric and colorimetric procedures for the assay of serum glutamic-oxaloacetic transaminase (SGOT) activity are assessed. Directions are given for the preparation of reagents and for a procedure that corrects the errors in the spectrophotometric method without altering its basic simplicity. The colorimetric procedure employing dinitrophenylhydrazine showed poor accuracy due, in part, to a large and variable blank.

The proposed procedure eliminates the troublesome group of analytic results, "borderline" measurements, a source of diagnostic uncertainty.

Submitted on January 8, 1962




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