Clinical Chemistry
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Clinical Chemistry 8: 370-377, 1962;
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Clinical Chemistry, Vol 8, 370-377, Copyright © 1962 by the American Association for Clinical Chemistry

Determination of Urine Dopamine

Fritz Bischoff 1 and Adolfo Torres 1

1 Chemical Laboratory, Santa Barbara Cottage Hospital Research Institute, Santa Barbara, Calif.

The Duliere and Raper conversion of dopamine to 5,6-hydroxyindole is applied to urinary dopamine, which is concentrated and recovered by adsorption (pH 8.5-9.0) on and elution with dilute acetic acid from alumina. The spectrofluorometric readings are made at pH 5.3, with activation and fluorescence at 320 and 375 mµ, respectively. Proportionality between high and low internal standards is achieved when the 5,6-hydroxyindole reaction proceeds for 20 hours at room temperature. The increase of fluorescence with time adds specificity to the test. The recovery of standards added before adsorption on alumina compared with standards added after elution was 98 per cent.

Urinary dopamine was determined in normal individuals asleep and awake and in those with a number of pathologic conditions. The mean values were decreased in groups with Parkinsonism, diabetes, and cirrhosis of the liver. The study included 2 patients with pheochromocytoma.

Submitted on February 2, 1962




The following articles in journals at HighWire Press have cited this article:


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 Arch Gen PsychiatryHome page
T. J. Crowley, M. M. Hoehn, C. O. Rutledge, M. A. Stallings, R. K. Heaton, S. Sundell, and D. Stilson
Dopamine Excretion and Vulnerability to Drug-Induced Parkinsonism: Schizophrenic Patients
Arch Gen Psychiatry, January 1, 1978; 35(1): 97 - 104.
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Copyright © 1962 by the American Association for Clinical Chemistry.