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Clinical Chemistry 8: 378-388, 1962;
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Clinical Chemistry, Vol 8, 378-388, Copyright © 1962 by the American Association for Clinical Chemistry

Interferences in the Colorimetric Determination of Glucuronic Acid in Blood Serum

Robert E. Mosher 1

1 Department of Research, Providence Hospital, Detroit 8, Mich.

The naphthoresorcinol reaction for the colorimetric determination of glucuronic acid has been investigated. The interference of glucose has been minimized by incorporating glucose, to approximate serum levels, in the glucuronolactone standards and adhering to exact analytic conditions. The colored product is extracted into beta,beta dichloroethyl ether and the absorbance is measured at 580 mµ.

Submitted on December 30, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.