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Clinical Chemistry 8: 502-508, 1962;
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Clinical Chemistry, Vol 8, 502-508, Copyright © 1962 by the American Association for Clinical Chemistry

Study of a Differential Demasking Technic for Serum Zinc

Louis A. Williams 1, Jerry S. Cohen 1, and Bennie Zak 1

1 Department of Pathology, Wayne State University College of Medicine and Detroit Receiving Hospital, Detroit, Mich.

A differential decomplexation technic for the determination of serum zinc in filtrates was studied. It involved the masking of zinc, copper, and iron by the addition of cyanide to a buffered aliquot of an acid filtrate, followed first by the destruction of the excess cyanide and then the cyanide attached to zinc. The release of ionic zinc allowed reaction with the color reagent to take place. Quantitation with internal blanking was thereby accomplished before interference by other cations became possible.






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Copyright © 1962 by the American Association for Clinical Chemistry.