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Clinical Chemistry 8: 570-578, 1962;
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Clinical Chemistry, Vol 8, 570-578, Copyright © 1962 by the American Association for Clinical Chemistry

A New Diazo Method for the Determination of Bilirubin

Royden N. Rand 1 and Alfred di Pasqua 1

1 Department of Laboratories, St. Mary's Hospital, Rochester, N. Y.

A new technic for the determination of total bilirubin is reported. The substitution of 2,4 dichloroaniline for sulfanilic acid results in a very stable diazotized reagent which reacts quickly with bilirubin in plasma or serum. Comparison with a sulfanilic acid procedure indicates that similar analytic values are obtained with this technic; short quality control studies indicate satisfactory precision was obtained.

Submitted on August 19, 1962




The following articles in journals at HighWire Press have cited this article:


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H. E. Rugstad, S. H. Robinson, C. Yannoni, and A. H. Tashjian Jr.
Transfer of Bilirubin Uridine Diphosphate-Glucuronyltransferase to Enzyme-Deficient Rats
Science, October 30, 1970; 170(3957): 553 - 555.
[Abstract] [PDF]


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Copyright © 1962 by the American Association for Clinical Chemistry.