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Clinical Chemistry 8: 593-597, 1962;
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Clinical Chemistry, Vol 8, 593-597, Copyright © 1962 by the American Association for Clinical Chemistry

Changes in Ammonia Values of Protein-free Filtrates of Blood During Storage in the Frozen State

Charles E. Wilder 1

1 Medical Services of the Lemuel Shattuck Hospital and the Department of Medicine Tufts University School of Medicine, Boston 30, Mass.

Protein-free filtrates of blood treated with trichloracetic acid were divided into two or three parts. The ammonia concentration of one of these was determined immediately by the ninhydrin method described by Nathan and Rodkey (1). The others were kept frozen for periods of 1-8 days and then thawed and analyzed. The blood ammonia concentrations calculated from these filtrate values were compared. A significant change in ammonia level during frozen storage was demonstrated.

Submitted on June 23, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.