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Clinical Chemistry 8: 616-625, 1962;
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Clinical Chemistry, Vol 8, 616-625, Copyright © 1962 by the American Association for Clinical Chemistry

Estimation of Low-Density Lipoprotein Immunoprecipitates in Uniform Drops of Serum

R. L. Searcy 1, T. M. Asher 1, and L. M. Bergquist 1

1 Departments of Pathology, Los Angeles County General Hospital (Unit 2), California College of Medicine, and Research Department, Hyland Laboratories, Los Angeles, Calif.

An immunochemical technic has been found to be specific, efficient and reproducible in removing lipoproteins of density less than 1.063 gm./ml. from measured drops of human serum. Estimations of microcapillary columns of immunoprecipitate compare favorably with ultracentrifugal analysis of Sf 0-400 lipoproteins. An equation derived from these data converts millimeters of lipoprotein precipitate into milligrams lipoprotein Sf 0-400 per 100 ml. serum. A highly significant difference was obtained between mean lipoprotein precipitate levels of a clinically healthy population and those of a group of patients with coronary artery disease or diabetes mellitus.

Submitted on August 23, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.