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Clinical Chemistry 8: 647-653, 1962;
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Clinical Chemistry, Vol 8, 647-653, Copyright © 1962 by the American Association for Clinical Chemistry

Determination of Organic Iodine Compounds in Serum

II. Chromatographic Studies of Exogenous Organic Iodine Compounds Affecting the PBI and BEI

Vincent J. Pileggi 1, Richard J. Henry 1, Milton Segalove 1, and George C. Hamill 2

1 Bio-Science Laboratories, 12330 Santa Monica Blvd., Los Angeles 25, California.
2 Major, USAF (MC), U.S.A.F. Hospital Maxwell (AU), Maxwell Air Force Base, Alabama.

RI — values of organic iodine compounds which interfere with the determination of serum protein-bound iodine (PBI) and butanol-extractable iodine (BEI) levels were obtained for the solvent system 2-butanol-4% NH4OH (3:1) utilizing descending paper chromatography. The feasibility of employing this system for identification of exogenous organic iodine in patients' sera was investigated. In general, the demonstration of these compounds or their metabolites was achieved only in sera obtained shortly after administration. This was due mainly to sensitivity limitations in the detection of the compounds on paper. Some data on the duration of interference with the PBI and BEI were also obtained for the radiopaque dyes Telepaque, Hypaque, Salipix, and Orabilex.

Submitted on November 16, 1962






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Copyright © 1962 by the American Association for Clinical Chemistry.