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Clinical Chemistry 8: 660-664, 1962;
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Clinical Chemistry, Vol 8, 660-664, Copyright © 1962 by the American Association for Clinical Chemistry

Oxidation of Homogentisic Acid by Nitrous Acid and its Interference in the Determination of 5-Hydroxyindoleacetic Acid

Richard B. Davis 1

1 Department of Medicine, University of Minnesota Medical School and University of Minnesota Hospitals, Minneapolis, Minn.

Oxidation of homogentisic acid to the corresponding quinone occurs in the presence of reagents used to develop the violet chromophore with 5-hydroxyindoleacetic acid(5-HIAA). With amounts of homogentisic acid well below those excreted in alkaptonuria, significant interference in the development of the violet chromophore of 5-HIAA occurs. The mechanism of this interference is discussed. Compounds related to homogentisic acid do not cause interference. Using the simple conditions described, a rapid oxidation of homogentisic acid is effected. Absorption of the corresponding quinone is stable and linear with relation to the initial concentration of homogentisic acid over a 10-fold range of concentration.

Submitted on December 7, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.