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Clinical Chemistry 9: 102-108, 1963;
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Clinical Chemistry, Vol 9, 102-108, Copyright © 1963 by the American Association for Clinical Chemistry

A New Simple Semimicro Method for Colorimetric Determination of Urea

J. J. Coulombe 1 and L. Favreau 1

1 Hotel-Dieu Hospital Biochemical Laboratory and St.-Vincent-de-Paul Hospital Biochemical Laboratory, Sherbrooke, Québec, Canada.

A new simple and rapid method for quantitative semimicro determination of blood urea is presented. Diacetyl monoxime and thiosemicarbazide form in acid medium with urea a red color complex with a maximum adsorption at 530 mµ stable at room temperature and requiring 0.2 ml. of 1:10 blood filtrate.

Submitted on January 22, 1962




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