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Clinical Chemistry 9: 27-30, 1963;
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Clinical Chemistry, Vol 9, 27-30, Copyright © 1963 by the American Association for Clinical Chemistry

Determination of Serum Amino Acids

Robert D. Rapp 1

1 Department of Biochemistry, the Reading Hospital, Reading, Pa.

The reaction of 1-fluoro-2,4 dinitrobenzene with amino acids was investigated as an assay procedure for serum amino acids. An aliquot of a tungistic acid filtrate of serum is buffered at H 10, mixed with the buffered reagent, and heated at 56° for 15 min. The resultant color is read at 420 mµ.

Submitted on January 3, 1962






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Copyright © 1963 by the American Association for Clinical Chemistry.