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Clinical Chemistry 9: 70-78, 1963;
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Clinical Chemistry, Vol 9, 70-78, Copyright © 1963 by the American Association for Clinical Chemistry

Stationary Phase as Color Reagent in Glass Paper Chromatography of Estrogens

Emanuel Epstein 1 and Bennie Zak 1

1 Departments of Pathology, St. Joseph Mercy Hospital, Pontiac, Mich., and Wayne State University College of Medicine and Detroit Receiving Hospital, Detroit, Mich.

A new technic has been described for the chromatographic separation of estrogens. It includes the use of a dual-purpose system where the impregnating material of the stationary phase (p-toluene sulfonic acid) is used as a color reagent after the separation is complete. This novel approach precludes the need for a spraying or dipping step to achieve the desired color reaction. Quantitation by densitometry and fluorescence is also detailed.

Submitted on October 6, 1961






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Copyright © 1963 by the American Association for Clinical Chemistry.