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Clinical Chemistry 9: 79-90, 1963;
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Clinical Chemistry, Vol 9, 79-90, Copyright © 1961 by the American Association for Clinical Chemistry

Detection and Quantitative Determination of Glycine with Pyridine and p-Nitrobenzoyl Chloride

Charles J. Umberger 1 and Frank F. Fiorese 1

1 Hospital for Special Surgery-Philip D. Wilson Research Foundation, affiliated with New York Hospital-Cornell University Medical Center, New York, N. Y.

Glycine in quantities as small as 0.5 µg. can be detected as an orange-red color by a specific reaction with p-nitrobenzoyl chloride and pyridine. The procedure can be used both as a qualitative spot test and as a sensitive quantitative method. In a discussion of the reaction mechanism, it is suggested that the color is due to azlactone formation.






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Copyright © 1963 by the American Association for Clinical Chemistry.