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Clinical Chemistry 9: 91-96, 1963;
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Clinical Chemistry, Vol 9, 91-96, Copyright © 1963 by the American Association for Clinical Chemistry

Colorimetric Method for Hippuric Acid

Charles J. Umberger 1 and Frank F. Fiorese 1

1 Hospital for Special Surgery-Philip D. Wilson Research Foundation, affiliated with New York Hospital, Cornell University Medical Center.

Hippuric acid dissolved in pyridine producesa red-orange color upon the addition of benzenesulfonyl chloride. The color has a stable absorbance at 380 mµ. It is sensitive in microgram quantities of hippuric acid and follows the Beer-Lambert Law over a wide range of concentrations. The mechanism of the color reaction is described.

Submitted on November 3, 1961






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Copyright © 1963 by the American Association for Clinical Chemistry.