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Clinical Chemistry 9: 360-364, 1963;
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Clinical Chemistry, Vol 9, 360-364, Copyright © 1963 by the American Association for Clinical Chemistry

Direct Determination of Calcium in Icteric Serum

B. Fingerhut 1 and H. Miller 1

1 Institute of Pathology, Kings County Hospital Center, Brooklyn 3, N. Y.

A rapid method suitable for the direct titrimetric determination of calcium in icteric sera is presented. The major portion of serum chromogens are oxidized to colorless products by either a mixture of hydrogen peroxide and potassium ferricyanide or by sodium hypochlorite. Ethylenediamine tetraacetic acid (EDTA) is the titrant used and either calcein or Cal-Red is suitable as the specific calcium indicator. Results compare favorably with those obtained by the method of Clark and Collip.

Submitted on March 22, 1962






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Copyright © 1963 by the American Association for Clinical Chemistry.