| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 9, 391-399, Copyright © 1963 by the American Association for Clinical Chemistry
1 Biophysics Research Laboratory of the Division of Medical Biology, Department of Medicine, Harvard Medical School and Peter Bent Brigham Hospital, Boston, Mass.
Serum LDH activities assayed spectrophotometrically by the "forward" (lactate + DPN to pyruvate + DPNH + H) method have linear reaction rates, activity proportional to the amount of serum added, and a reproducibility of 1.3%. The reagent mixture can be stored frozen for 6 months and in the lyophilized state indefinitely.
Poor separation of normal from abnormal serum LDH activities results from the nonlinear calibration curves and decreasing reaction rates of the "backward" spectro-photometric assay. The dinitrophenylhydrazine colorimetric assay is deemed unsuitable for clinical use because large variations in activity result from minor variations in the reagent calibration curve, and because colorimetric activities are inaccurate as compared to activities obtained with the "forward" spectrophotometric assay.
Submitted on June 18, 1962
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  C R Valeri, H Macgregor, G Ragno, N Healey, J Fonger, and S F Khuri Effects of centrifugal and roller pumps on survival of autologous red cells in cardiopulmonary bypass surgery Perfusion, September 1, 2006; 21(5): 291 - 296. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Yasuda, M. Yamaya, T. Sasaki, D. Inoue, K. Nakayama, M. Yamada, M. Asada, M. Yoshida, T. Suzuki, H. Nishimura, et al. Carbocisteine inhibits rhinovirus infection in human tracheal epithelial cells Eur. Respir. J., July 1, 2006; 28(1): 51 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Bemis and R. F. Seegal PCB-Induced Inhibition of the Vesicular Monoamine Transporter Predicts Reductions in Synaptosomal Dopamine Content Toxicol. Sci., August 1, 2004; 80(2): 288 - 295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kemp, J. Donovan, H. Higham, and J. Hooper Biochemical markers of myocardial injury Br. J. Anaesth., July 1, 2004; 93(1): 63 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Moshynska, K. Sankaran, P. Pahwa, and A. Saxena Prognostic Significance of a Short Sequence Insertion in the MCL-1 Promoter in Chronic Lymphocytic Leukemia J Natl Cancer Inst, May 5, 2004; 96(9): 673 - 682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Powell and R. M. Jackson Mitochondrial complex I, aconitase, and succinate dehydrogenase during hypoxia-reoxygenation: modulation of enzyme activities by MnSOD Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L189 - L198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Okolo, T. Wong, M. W. Moody, and T. D. Nguyen Effects of bile acids on dog pancreatic duct epithelial cell secretion and monolayer resistance Am J Physiol Gastrointest Liver Physiol, November 1, 2002; 283(5): G1042 - G1050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Thohan, M. C. Zurich, H. Chung, M. Weiner, A. S. Kane, and G. M. Rosen Tissue Slices Revisited: Evaluation and Development of a Short-Term Incubation for Integrated Drug Metabolism Drug Metab. Dispos., October 1, 2001; 29(10): 1337 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ishizuka, M. Yamaya, T. Suzuki, K. Nakayama, M. Kamanaka, S. Ida, K. Sekizawa, and H. Sasaki Acid Exposure Stimulates the Adherence of Streptococcus pneumoniae to Cultured Human Airway Epithelial Cells . Effects on Platelet-Activating Factor Receptor Expression Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 459 - 468. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. K. Roche, C. A. P. Martins, R. Cosme, R. Fayer, and R. L. Guerrant Transforming Growth Factor beta 1 Ameliorates Intestinal Epithelial Barrier Disruption by Cryptosporidium parvum In Vitro in the Absence of Mucosal T Lymphocytes Infect. Immun., October 1, 2000; 68(10): 5635 - 5644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R C Gupta, J T Goad, D Milatovic, and W-D Dettbarn Cholinergic and noncholinergic brain biomarkers of insecticide exposure and effects Human and Experimental Toxicology, May 1, 2000; 19(5): 297 - 308. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Hugel, M. Horn, M. de Groot, H. Remkes, C. Dienesch, K. Hu, G. Ertl, and S. Neubauer Effects of ACE inhibition and beta -receptor blockade on energy metabolism in rats postmyocardial infarction Am J Physiol Heart Circ Physiol, December 1, 1999; 277(6): H2167 - H2175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. M. Siow, H. Sato, D. S. Leake, J. D. Pearson, S. Bannai, and G. E. Mann Vitamin C Protects Human Arterial Smooth Muscle Cells Against Atherogenic Lipoproteins : Effects of Antioxidant Vitamins C and E on Oxidized LDL–Induced Adaptive Increases in Cystine Transport and Glutathione Arterioscler Thromb Vasc Biol, October 1, 1998; 18(10): 1662 - 1670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. OHRUI, M. YAMAYA, K. SEKIZAWA, N. YAMADA, T. SUZUKI, M. TERAJIMA, S. OKINAGA, and H. SASAKI Effects of Rhinovirus Infection on Hydrogen Peroxide- induced Alterations of Barrier Function in the Cultured Human Tracheal Epithelium Am. J. Respir. Crit. Care Med., July 1, 1998; 158(1): 241 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Terajima, M. Yamaya, K. Sekizawa, S. Okinaga, T. Suzuki, N. Yamada, K. Nakayama, T. Ohrui, T. Oshima, Y. Numazaki, et al. Rhinovirus infection of primary cultures of human tracheal epithelium: role of ICAM-1 and IL-1beta Am J Physiol Lung Cell Mol Physiol, October 1, 1997; 273(4): L749 - L759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mukhin, L. Fan, and A. I. Faden Activation of Metabotropic Glutamate Receptor Subtype mGluR1 Contributes to Post-Traumatic Neuronal Injury J. Neurosci., October 1, 1996; 16(19): 6012 - 6020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Harvey, G. Healing, I. Major, M. McFarlane, K. Purdy, O. Olatunde, M. Garcia Conesa, D. Everett, and A. Cockburn Glucocorticoid amelioration of nephrotoxicity: a study of cephaloridine- methylprednisolone interaction in the rat Human and Experimental Toxicology, July 1, 1995; 14(7): 554 - 561. [Abstract] [PDF]
|
|
|
|
 |
|
 H. J. Saranchak and S. H. Bernstein A New Diagnostic Test for Acute Myocardial Infarction: The Detection of Myoglobinuria by Radioimmunodiffusion Assay JAMA, June 3, 1974; 228(10): 1251 - 1255. [Abstract] [PDF]
|
|
|
|
 |
|
 N. S. Lawson, M. D. Waters, J. J. Amato, L. J. Billy, and E. Arsenal Serum Enzymes in Experimental Ballistic Injury to Extremities Arch Surg, December 1, 1971; 103(6): 741 - 745. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Wuntch, R. F. Chen, and E. S. Vesell Lactate Dehydrogenase Isozymes: Kinetic Properties at High Enzyme Concentrations Science, January 2, 1970; 167(3914): 63 - 65. [Abstract] [PDF]
|
|
|
|
|
|
R. A. DeRemee Serum Lactic Dehydrogenase Activity and Diffuse Interstitial Pneumonitis JAMA, June 24, 1968; 204(13): 1193 - 1195. [Abstract] [PDF]
|
|
|
|
 |
|
 A. KONTTINEN SERUM LACTATE DEHYDROGENASE AND PULMONARY EMBOLISM Ann Intern Med, June 1, 1967; 66(6): 1291 - 1295. [Abstract] [PDF]
|
|
|
|
|
|
E. AMADOR, L. E. DORFMAN, and W. E. C. WACKER Urinary Alkaline Phosphatase and LDH Activities in the Differential Diagnosis of Renal Disease Ann Intern Med, January 1, 1965; 62(1): 30 - 40. [Abstract] [PDF]
|
|
|
|
|
|
W. E. C. Wacker, L. E. Dorfman, and E. Amador Urinary Lactic Dehydrogenase Activity: IV. Screening Test for Detection of Renal Disease Dissociated and in Association With Arterial Hypertension JAMA, May 18, 1964; 188(7): 671 - 676. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
