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Clinical Chemistry 43: 195-196, 1997;
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(Clinical Chemistry. 1997;43:195-196.)
© 1997 American Association for Clinical Chemistry, Inc.

Letters

Automated Immunoassay of Cardiac Troponin I in Serum Evaluated

Mauro Panteghinia, Roberto Bonora and Franca Pagani

1st Lab. Clin. Chem., Clin. Chem. and Enzymol. Section, Spedali Civili, 25125 Brescia, Italy
a Author for correspondence.


To the Editor:

We evaluated the Opus Troponin I assay (Behring Diagnostics, Westwood, MA), a two-site sandwich, fluorogenic ELISA that uses two goat polyclonal antibodies directed against different protein segments unique to cardiac troponin I (cTnI) (1). Pipetting, incubations, measurements, and data-reduction steps are performed on the Opus analyzer; the first test result requires 20 min. The assay measures concentrations of cTnI in serum as great as ~135 µg/L.

The calibration appeared to be stable for at least 4 weeks. Serial dilution of a human serum sample with a high concentration of cTnI showed no significant curvature when the curve obtained was tested for linearity [quadratic regression: y = -0.25 + 96.1x + 6.31x2, with the coefficient of x2 not significantly different from 0 (P = 0.27)] (2). Linear regression analysis of these data confirmed the high linearity of the response (r = 0.9998). The minimum detectable cTnI concentration, assessed by . . . [Full Text of this Article]


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