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Clinical Chemistry 44: 186, 1998;
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(Clinical Chemistry. 1998;44:186.)
© 1998 American Association for Clinical Chemistry, Inc.

Letters

When Is an Indole Not an Indole?

John A. Martin, and Kathryn Brownbill

Dept. of Clin. Chem., Royal Bolton Hosp., Minerva Rd., Farnworth, Bolton BL4 OJR, UK


To the Editor:

An indole is not an indole when it is a naphthalene derivative!

We routinely measure 5-hydroxyindoleacetic acid (5HIAA) by a long-established method using nitrosonaphthol, as first described by Udenfriend in 1955 (1), but with various modifications to improve specificity (2). The profession's elder statesmen may well recall the method of Hanson and Serin (3) using Erlich's reagent.

Although the Udenfriend method is very specific for 5-hydroxyindoles, it is subject to interference, probably the most documented . . . [Full Text of this Article]


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