Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 44: 188-190, 1998;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cole, D. E. C.
Right arrow Articles by Evrovski, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cole, D. E. C.
Right arrow Articles by Evrovski, J.
Related Collections
Right arrow Nutrition
Right arrow Endocrinology and Metabolism
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 1998;44:188-190.)
© 1998 American Association for Clinical Chemistry, Inc.

Letters

Simplified Simultaneous Assay of Total Plasma Homocysteine and Methionine by HPLC and Pulsed Integrated Amperometry

David E. C. Cole1, Denis C. Lehotay, and Jovan Evrovski

1 Depts. of Lab. Med. & Pathobiol., Med. and Paediatr. (Genetics), Univ. of TorontoBanting Inst., Rm. 415, 100 College St., Toronto, ON M5G 1L5Canada


To the Editor:

Measurement of total plasma homocysteine (tHcy) can be a useful adjunct in the diagnosis of cobalamin or folate deficiency and is emerging as an independent predictor in many vaso-occlusive diseases (1). As clinical interest in this metabolite grows, the demand for simple and efficient methods of determination has increased. In some situations, a methionine-loading test may be conducted to evaluate homocysteine catabolism, but methionine is rarely measured concomitantly, because it usually requires a different assay methodology altogether. In homocystinuria caused by cystathionine beta-synthase deficiency, circulating methionine is often increased, whereas homocystinuria resulting from a relative deficiency of the remethylation pathway is characterized by hypomethioninemia (2).

In our previously reported serum assay for tHcy (3), we used the DX-500 Ion Chromatograph (Dionex Canada), outfitted with two pumps (in parallel), valves, and two columns (a 4 x 50 mm OmniPac PCX-500 precolumn and a 4 x 250 mm OmniPac PCX-500 analytical column) plumbed in series to permit "heart-cut" trapping of tHcy (4). However, with the ED40 electrochemical detector set for pulsed integrated amperometry (PIA) mode, any compound with a reduced sulfur atom, including methionine, will generate a signal proportional to concentration (5).

In our initial procedure, the disulfide reduction procedure with sodium borohydride (NaBH4) (6) was the most labor-intensive step . . . [Full Text of this Article]


Acknowledgments


Footnotes


References




The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Clin. Nutr.Home page
L. A Houghton, K. L Sherwood, R. Pawlosky, S. Ito, and D. L O'Connor
[6S]-5-Methyltetrahydrofolate is at least as effective as folic acid in preventing a decline in blood folate concentrations during lactation.
Am. J. Clinical Nutrition, April 1, 2006; 83(4): 842 - 850.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
M. Di Buono, L. J. Wykes, D. E. C. Cole, R. O. Ball, and P. B. Pencharz
Regulation of Sulfur Amino Acid Metabolism in Men in Response to Changes in Sulfur Amino Acid Intakes
J. Nutr., March 1, 2003; 133(3): 733 - 739.
[Abstract] [Full Text] [PDF]

Home page
 Arch Intern MedHome page
L. J. Langman, J. G. Ray, J. Evrovski, E. Yeo, and D. E.C. Cole
Hyperhomocyst(e)inemia and the Increased Risk of Venous Thromboembolism: More Evidence From a Case-Control Study
Arch Intern Med, April 10, 2000; 160(7): 961 - 964.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
D. E.C. Cole, H. J. Ross, J. Evrovski, L. J. Langman, S. E.S. Miner, P. A. Daly, and P.-Y. Wong
Correlation between total homocysteine and cyclosporine concentrations in cardiac transplant recipients
Clin. Chem., November 1, 1998; 44(11): 2307 - 2312.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Association for Clinical Chemistry.