Clinical Chemistry
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Clinical Chemistry 45: 407-409, 1999;
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(Clinical Chemistry. 1999;45:407-409.)
© 1999 American Association for Clinical Chemistry, Inc.

Technical Briefs

Polyethylene Glycol Increases the Detection of Anti-Thyrotropin Receptor Antibodies by a Radioreceptor Assay

Yukihiko Watanabe1, Hisato Tada1, Yoh Hidaka2, Toru Takano1, Keiko Takeoka1, Shuji Fukata2, Kanji Kuma2 and Nobuyuki Amino1,a

1 Department of Laboratory Medicine, Osaka University Medical School, Suita 565-0871, Japan, and
2 Kuma Hospital, Kobe 650-0011, Japan;
a author for correspondence: fax 81-6-879-3239, e-mail namino@labo. qjmed.osaka-u.ac.jp

Autoantibodies to the thyrotropin (TSH) receptor (TSHR) are the hallmark of the autoimmune response to the thyroid gland and are responsible for thyrotoxicosis in Graves disease (1). Unlike other autoantibodies, there is no direct assay for TSHR antibodies. Instead, these antibodies are detected either by their ability to inhibit binding of radiolabeled TSH to receptors or by a bioassay of TSHR activation (2)(3). The most widely used assay is a radioreceptor assay, which uses solubilized porcine TSHR, in which the antibody is called TSH-binding inhibitory immunoglobulin (TBII). Serum TBII is easily assayed with a commercially available kit; however, 5–10% of patients with Graves disease show a negative reaction perhaps related to inadequacies of the assay (4).

Addition of polyethylene glycol (PEG) enhances cAMP production induced by a patient's IgG, but not by TSH, forskolin, or GTP{gamma}S in an in vitro bioassay for thyroid stimulators (5), suggesting that the enhancement occurs at the step of antibody-receptor interaction. . . . [Full Text of this Article]


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The following articles in journals at HighWire Press have cited this article:


Home page
 J. Clin. Endocrinol. Metab.Home page
N. Amino, H. Tada, and Y. Hidaka
Screening for Postpartum Thyroid Dysfunction in the General Population Is Beneficial
J. Clin. Endocrinol. Metab., June 1, 1999; 84(6): 1813 - 1816.
[Full Text]


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