Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 45: 683-685, 1999;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eastman, J. W.
Right arrow Articles by Cunningham, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eastman, J. W.
Right arrow Articles by Cunningham, G.
Related Collections
Right arrow Proteomics and Protein Markers
Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 1999;45:683-685.)
© 1999 American Association for Clinical Chemistry, Inc.

Technical Briefs

Distribution of Hemoglobin F, A, S, C, E, and D Quantities in 4 Million Newborn Screening Specimens

John W. Eastman1,1, Fred Lorey2, John Arnopp2, Robert J. Currier2, John Sherwin1 and George Cunningham2

1 California Department of Health Services, Genetic Disease Laboratory, 700 Heinz Ave., Suite 100, Berkeley, CA 94710, and
2 California Department of Health Services, Genetic Disease Branch, Berkeley, CA 94704;

The California newborn screening program requires that all newborns be screened for selected hemoglobinopathies. Dried blood spot (DBS) specimens are analyzed using an automated 2-min cation-gradient HPLC method that is sensitive and specific (1)(2). The standardized method selected by the State allows the screening program to match the quantitative analytical performance of the assay at eight private contract laboratories and a central laboratory. Information technology is designed to derive phenotypes automatically and to use quantitative acceptability limits for quality control and proficiency testing.

The distribution of hemoglobin (Hb) quantities determined by cation-exchange HPLC in cord blood specimens has been published previously (3)(4). We have determined the frequency distributions for the analysis of DBS specimens using the rapid HPLC screening method. In this study, we examined screening data reported on 4 million nontransfused newborns tested within 2 days of birth. The frequency distributions were determined for the percentages of Hb concentrations in 14 phenotypes containing Hbs F, A, S, C, E, and D. The frequency distributions are available on request. The medians only are given in Table 1 .


View this table:
[in this window]
[in a new window]
  		Table 1. Median percentages of Hb concentrations in newborn DBS specimens by rapid cation-exchange HPLC.

The percent concentrations in the current study are lower than the published cord blood data by 15% for all Hb F and by a median 36% for the Hb in other hetero- and homozygous patterns (range, 22–40%). This may be caused by differences in the HPLC methodology and by chemical degradation of Hb during formation of . . . [Full Text of this Article]


Footnotes


References




The following articles in journals at HighWire Press have cited this article:


Home page
 Am J Clin PatholHome page
D. D. Mais, R. D. Gulbranson, and D. F. Keren
The Range of Hemoglobin A2 in Hemoglobin E Heterozygotes as Determined by Capillary Electrophoresis
Am J Clin Pathol, July 1, 2009; 132(1): 34 - 38.
[Abstract] [Full Text] [PDF]

Home page
 Annals of Clinical & Laboratory ScienceHome page
L. B. Thomas, S. J. Agosti, M. A. Man, and S. M. Mastorides
Screening for Hemoglobinopathies During Routine Hemoglobin A1c Testing Using the Tosoh G7 Glycohemoglobin Analyzer
Ann. Clin. Lab. Sci., January 1, 2007; 37(3): 251 - 255.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Joutovsky, J. Hadzi-Nesic, and M. A. Nardi
HPLC Retention Time as a Diagnostic Tool for Hemoglobin Variants and Hemoglobinopathies: A Study of 60000 Samples in a Clinical Diagnostic Laboratory
Clin. Chem., October 1, 2004; 50(10): 1736 - 1747.
[Abstract] [Full Text] [PDF]

Home page
 Arch Pediatr Adolesc MedHome page
D. B. Richardson, S. Wing, F. Lorey, and I. Hertz-Picciotto
Adult Hemoglobin Levels at Birth and Risk of Sudden Infant Death Syndrome
Arch Pediatr Adolesc Med, April 1, 2004; 158(4): 366 - 371.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Association for Clinical Chemistry.