| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
Departments of
1
Chemical Pathology and
2
Obstetrics and Gynecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR
a address correspondence to this author at: Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Room 38023, 1/F Clinical Sciences Building, 30-32 Ngan Shing Street, Shatin, New Territories, Hong Kong SAR; fax 852-2194-6171, e-mail loym@cuhk.edu.hk
 |
Introduction |
|---|
Pregnant women attending the Prenatal Diagnosis Unit at the Department
of Obstetrics and Gynecology, Prince of Wales Hospital, Hong Kong were
recruited with informed consent. The study was approved by the Clinical
Research Ethics Committee. Women early and late in their pregnancies
(n = 21 and 37, respectively) were recruited in this study. The
mean gestational ages of the subjects in early and late pregnancies
were 16 weeks (range, 11–19 weeks) and 33 weeks (range, 26–40 weeks),
respectively. All early-pregnancy samples were obtained before any
invasive procedure. On the other hand, late-pregnancy samples were
collected either from women who had invasive procedures in early
pregnancy (n = 21) or from women who did not have any prenatal
invasive procedure (n = 16). All plasma samples were harvested
within 30 min from EDTA-blood samples as described previously
(1). Total RNA from plasma samples was isolated with the
Trizol LS Reagent (Life
|
Acknowledgments |
|---|
|
References |
|---|
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  S. Huang, H. Li, X. Ding, and C. Xiong Presence and Characterization of Cell-Free Seminal RNA in Healthy Individuals: Implications for Noninvasive Disease Diagnosis and Gene Expression Studies of the Male Reproductive System Clin. Chem., November 1, 2009; 55(11): 1967 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. F Wright and L. S Chitty Cell-free fetal DNA and RNA in maternal blood: implications for safer antenatal testing BMJ, July 6, 2009; 339(jul06_2): b2451 - b2451. [Full Text]
|
|
|
|
 |
|
 E C W Hung, R W K Chiu, and Y M D Lo Detection of circulating fetal nucleic acids: a review of methods and applications J. Clin. Pathol., April 1, 2009; 62(4): 308 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. F. Wright and H. Burton The use of cell-free fetal nucleic acids in maternal blood for non-invasive prenatal diagnosis Hum. Reprod. Update, January 1, 2009; 15(1): 139 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Dennis Lo and R. W. K. Chiu Noninvasive Prenatal Diagnosis of Fetal Chromosomal Aneuploidies by Maternal Plasma Nucleic Acid Analysis Clin. Chem., March 1, 2008; 54(3): 461 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rani, M. Clynes, and L. O'Driscoll Detection of Amplifiable mRNA Extracellular to Insulin-Producing Cells: Potential for Predicting Beta Cell Mass and Function Clin. Chem., November 1, 2007; 53(11): 1936 - 1944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Purwosunu, A. Sekizawa, K. Koide, A. Farina, N. Wibowo, G. H. Wiknjosastro, S. Okazaki, H. Chiba, and T. Okai Cell-Free mRNA Concentrations of Plasminogen Activator Inhibitor-1 and Tissue-Type Plasminogen Activator Are Increased in the Plasma of Pregnant Women with Preeclampsia Clin. Chem., March 1, 2007; 53(3): 399 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C.K. Wong, R. W.K. Chiu, N. B.Y. Tsui, K.C. A. Chan, L. W. Chan, T. K. Lau, T. N. Leung, and Y.M. D. Lo Circulating Placental RNA in Maternal Plasma Is Associated with a Preponderance of 5' mRNA Fragments: Implications for Noninvasive Prenatal Diagnosis and Monitoring Clin. Chem., October 1, 2005; 51(10): 1786 - 1795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. B. Larrabee, K. L. Johnson, I. Peter, and D. W. Bianchi Presence of Filterable and Nonfilterable Cell-Free mRNA in Amniotic Fluid Clin. Chem., June 1, 2005; 51(6): 1024 - 1026. [Full Text] [PDF]
|
|
|
|
 |
|
 Y.M. D. Lo Recent Advances in Fetal Nucleic Acids in Maternal Plasma J. Histochem. Cytochem., March 1, 2005; 53(3): 293 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Gingeras, R. Higuchi, L. J. Kricka, Y.M. D. Lo, and C. T. Wittwer Fifty Years of Molecular (DNA/RNA) Diagnostics Clin. Chem., March 1, 2005; 51(3): 661 - 671. [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Larrabee, K. L. Johnson, C. Lai, J. Ordovas, J. M. Cowan, U. Tantravahi, and D. W. Bianchi Global Gene Expression Analysis of the Living Human Fetus Using Cell-Free Messenger RNA in Amniotic Fluid JAMA, February 16, 2005; 293(7): 836 - 842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Z. Bischoff, D. E. Lewis, and J. L. Simpson Cell-free fetal DNA in maternal blood: kinetics, source and structure Hum. Reprod. Update, January 1, 2005; 11(1): 59 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Gupta, W. Holzgreve, B. Huppertz, A. Malek, H. Schneider, and S. Hahn Detection of Fetal DNA and RNA in Placenta-Derived Syncytiotrophoblast Microparticles Generated in Vitro Clin. Chem., November 1, 2004; 50(11): 2187 - 2190. [Full Text] [PDF]
|
|
|
|
|
|
E. K.O. Ng, A. El-Sheikhah, R. W.K. Chiu, K.C. A. Chan, M. Hogg, R. Bindra, T. N. Leung, T. K. Lau, K. H. Nicolaides, and Y.M. D. Lo Evaluation of Human Chorionic Gonadotropin {beta}-Subunit mRNA Concentrations in Maternal Serum in Aneuploid Pregnancies: A Feasibility Study Clin. Chem., June 1, 2004; 50(6): 1055 - 1057. [Full Text] [PDF]
|
|
|
|
 |
|
 N B Y Tsui, S S C Chim, R W K Chiu, T K Lau, E K O Ng, T N Leung, Y K Tong, K C A Chan, and Y M D Lo Systematic micro-array based identification of placental mRNA in maternal plasma: towards non-invasive prenatal gene expression profiling J. Med. Genet., June 1, 2004; 41(6): 461 - 467. [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Dukes, L. M. Sullivan, D. Lewis, K. L. Johnson, D. W. Bianchi, J. L. Simpson, W. Holzgreve, S. Hahn, F. Z. Bischoff, and L. G. Jackson The Effect of the Elapsed Time Between Blood Draw and Processing on the Recovery of Fetal Cells From Maternal Blood Reproductive Sciences, April 1, 2004; 11(3): 154 - 165. [Abstract] [PDF]
|
|
|
|
|
|
C. B.M. Oudejans, A. T.J.J. Go, A. Visser, M. A.M. Mulders, B. A. Westerman, M. A. Blankenstein, and J. M.G. van Vugt Detection of Chromosome 21-encoded mRNA of Placental Origin in Maternal Plasma Clin. Chem., September 1, 2003; 49(9): 1445 - 1449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Costa, A. Benachi, M. Olivi, Y. Dumez, M. Vidaud, and E. Gautier Fetal Expressed Gene Analysis in Maternal Blood: A New Tool for Noninvasive Study of the Fetus Clin. Chem., June 1, 2003; 49(6): 981 - 983. [Full Text] [PDF]
|
|
|
|
|
|
R. W.K. Chiu, L. Y.S. Chan, N. Y.L. Lam, N. B.Y. Tsui, E. K.O. Ng, T. H. Rainer, and Y.M. D. Lo Quantitative Analysis of Circulating Mitochondrial DNA in Plasma Clin. Chem., May 1, 2003; 49(5): 719 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K.O. Ng, T. N. Leung, N. B.Y. Tsui, T. K. Lau, N. S. Panesar, R. W.K. Chiu, and Y.M. D. Lo The Concentration of Circulating Corticotropin-releasing Hormone mRNA in Maternal Plasma Is Increased in Preeclampsia Clin. Chem., May 1, 2003; 49(5): 727 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Ferguson-Smith Placental mRNA in maternal plasma: Prospects for fetal screening PNAS, April 15, 2003; 100(8): 4360 - 4362. [Full Text] [PDF]
|
|
|
|
|
|
E. K. O. Ng, N. B. Y. Tsui, T. K. Lau, T. N. Leung, R. W. K. Chiu, N. S. Panesar, L. C. W. Lit, K.-W. Chan, and Y. M. D. Lo From the Cover: mRNA of placental origin is readily detectable in maternal plasma PNAS, April 15, 2003; 100(8): 4748 - 4753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. V. Hviid In-Cell PCR Method for Specific Genotyping of Genomic DNA from One Individual in a Mixture of Cells from Two Individuals: A Model Study with Specific Relevance to Prenatal Diagnosis Based on Fetal Cells in Maternal Blood Clin. Chem., December 1, 2002; 48(12): 2115 - 2123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. B.Y. Tsui, E. K.O. Ng, and Y.M. D. Lo Stability of Endogenous and Added RNA in Blood Specimens, Serum, and Plasma Clin. Chem., October 1, 2002; 48(10): 1647 - 1653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K.O. Ng, N. B.Y. Tsui, N. Y.L. Lam, R. W.K. Chiu, S. C.H. Yu, S.C. C. Wong, E. S.F. Lo, T. H. Rainer, P. J. Johnson, and Y.M. D. Lo Presence of Filterable and Nonfilterable mRNA in the Plasma of Cancer Patients and Healthy Individuals Clin. Chem., August 1, 2002; 48(8): 1212 - 1217. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
