Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 46: 874-875, 2000;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Porter, W. H.
Right arrow Articles by Oeltgen, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Porter, W. H.
Right arrow Articles by Oeltgen, P.
Related Collections
Right arrow Drug Monitoring and Toxicology
(Clinical Chemistry. 2000;46:874-875.)
© 2000 American Association for Clinical Chemistry, Inc.

Technical Briefs

Interference by Glycolic Acid in the Beckman Synchron Method for Lactate: A Useful Clue for Unsuspected Ethylene Glycol Intoxication

William H. Porter2,a, Margaret Crellin2, Philip W. Rutter2 and Peter Oeltgen2,1

1 Lexington VA Hospital, Lexington, KY 40536

2 Department of Pathology and Laboratory Medicine, University
a author for correspondence: fax 606-257-8932, e-mail wporter@pop.uky.edu

In the differential evaluation of patients with high anion gap metabolic acidosis of unknown origin, lactate determinations are frequently performed. For patients who ingest ethylene glycol (present in antifreeze), the high anion gap metabolic acidosis is the result of the metabolism of ethylene glycol to glycolic acid (1)(2).

We encountered unusual lactate results, when measured on the Beckman LX 20 (Beckman Coulter), for two patients who had ingested ethylene glycol. Specifically, the lactate results were suppressed (i.e., no result) with an appended error message, "rate high". When these specimens were diluted threefold, measurable lactate values were obtained.

The Beckman lactate method is based on a lactate oxidase/peroxidase coupled reaction with endpoint determination. The lactate concentration is determined from the absorbance (A) measurement taken after reaction equilibrium has been established. To ensure an equilibrium steady state, a rate measurement is made during the expected steady-state portion of the measurement period. A reaction rate >=10 mA/min would indicate a nonequilibrium reaction condition and would lead to suppressed results and a "rate high" error flag.

We . . . [Full Text of this Article]


Footnotes


References




The following articles in journals at HighWire Press have cited this article:


Home page
 CMAJHome page
P. G. Brindley, M. S. Butler, G. Cembrowski, and D. N. Brindley
Falsely elevated point-of-care lactate measurement after ingestion of ethylene glycol
Can. Med. Assoc. J., April 10, 2007; 176(8): 1097 - 1099.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Association for Clinical Chemistry.