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Clinical Chemistry 46: 1207-1208, 2000;
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(Clinical Chemistry. 2000;46:1207-1208.)
© 2000 American Association for Clinical Chemistry, Inc.

Letters

Falsely Increased Values for Rabbit Immunoglobulin-based Nephelometric Immunoassays Attributable to Human Anti-Rabbit Antibodies

Anthony W. Butch

UCLA Medical Center, Department of Pathology, and Laboratory Medicine, 10833 Le Conte Ave., Mailroom A2-179 CHS, Los Angeles, CA 90095-1713, Fax 310-794-4864, E-mail abutch@mednet.ucla.edu


To the Editor:

A large number of serum proteins are routinely measured by automated immunonephelometric assays utilizing antisera derived from rabbits, goats, or sheep. Because this type of immunoassay uses polyclonal reagents derived from animals other than mice, it is free from interference by the human anti-mouse antibodies that commonly plague monoclonal antibody-based sandwich assays and competitive immunoassays (1). Nonspecific antibodies against murine immunoglobulins can be detected in the serum of a significant proportion of patients, with a prevalence estimate as high as 80% (2). The incidence of anti-rabbit antibodies in serum samples is considerably lower, with estimates between 0.1% and 5% (1)(3)(4)(5)(6). Interference from human anti-rabbit antibodies has been documented for two-site immunoassays and radioimmunoassays for several hormone assays as well as for creatine kinase MB (3)(4)(5)(6)(7)(8). It recently was reported that anti-rabbit antibodies produced falsely increased C-reactive protein values when measured by nephelometric . . . [Full Text of this Article]


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