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Clinical Chemistry 47: 1873-1874, 2001;
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(Clinical Chemistry. 2001;47:1873-1874.)
© 2001 American Association for Clinical Chemistry, Inc.

Letters

Real-Time PCR Assay with Fluorescent Hybridization Probes for Rapid Interleukin-6 Promoter (-174G->C) Genotyping

Thomas Bertsch1a, Wilma Zimmer1, Wendy Casarin1, Christof Denz2, Michael Quintel2 and Klaus Fassbender3

Departments of
1 Clinical Chemistry,
2 Anesthesiology, and
3 Neurology, University Hospital Mannheim of the University of Heidelberg, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, Germany

aAuthor for correspondence. Fax 49-621-383-3819; e-mail thomas.bertsch@ikc.ma.uni-heidelberg.de.


To the Editor:

Interleukin-6 (IL-6) is a central protein in the regulation of the inflammatory and immunologic response (1). A G->C polymorphism at position -174 has been associated with osteoporosis (2), juvenile rheumatoid arthritis (3), and atherosclerosis (4) with increased risks in the absence of the CC genotype.

Restriction fragment length polymorphism analysis has been used for IL-6 genotyping, but it is time-consuming and requires multiple manual steps. To improve throughput, we developed a rapid-cycle PCR method on the LightCyclerTM instrument (Roche Diagnostics) with fluorescent probe melting analysis. This assay is completed in 60 min.

DNA from 115 German Caucasians was extracted from . . . [Full Text of this Article]


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