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Clinical Chemistry 47: 1931-1933, 2001;
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(Clinical Chemistry. 2001;47:1931-1933.)
© 2001 American Association for Clinical Chemistry, Inc.

Abstracts of Oak Ridge Posters

Fast and Specific Hybridization Using Flow-Through Microarrays on Porous Metal Oxide

Rinie van Beuningen1a, Henk van Damme1, Piet Boender1, Niek Bastiaensen1, Alan Chan1 and Tim Kievits1

1 PamGene B.V., Burgemeester Loeffplein 70a, 5211RX Den Bosch, The Netherlands

aauthor for correspondence: fax 31-73-615-8081, e-mail RvBeuningen@PamGene.com

The advent of sequencing technologies and efforts in sequencing and analysis of polymorphic regions in various viruses, bacteria, and higher organisms, including humans, has led to a wealth of genetic information (1)(2). This information is used to relate genetic information to phenotypic effects, which is used to provide better tools in drug development and a better understanding of the biologic pathways involved in various rare as well as common diseases in humans. A tool in this type of analysis is parallel testing on the basis of microarrays. These arrays, sometimes referred to as "DNA-chips", usually consist of a flat surface with capture probes at specific positions (spots) directed toward the various targets that may be present in the sample.

The use of microarrays for genomic-based screening and the search for new genes has been well documented by scientific groups using systems (e.g., from Affymetrix and Sequenom) that have integrated many novel, microscale technologic developments (3)(4). The ability to either deposit directly, or synthesize in situ, hundreds or thousands of oligonucleotides on glass surfaces in subnanoliter volumes at high density allows for high-throughput simultaneous detection.

These first-generation microarrays, characterized by passive hybridization between targets and probes, are typically performed on planar surfaces. The fundamental problem caused by the concept of working on a basic two-dimensional surface has led many research groups to develop second-generation microarrays that seek to enhance the performance of this platform. The aim is more toward low costs, reproducibility of hybridization . . . [Full Text of this Article]


References




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