HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit an electronic Letter to the Editor about this paper Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Pieroni, L. Articles by Imbert-Bismut, F. Search for Related Content PubMed PubMed Citation Articles by Pieroni, L. Articles by Imbert-Bismut, F. Related Collections Molecular Diagnostics and Genetics Nutrition Drug Monitoring and Toxicology Hematology Endocrinology and Metabolism

Comparison of Bathophenanthroline Sulfonate and Ferene as Chromogens in Colorimetric Measurement of Low Hepatic Iron Concentration

¹ Laboratoire de Biochimie B,
² Service d’Anatomopathologie, and
³ Service d’Hépatogastroentérologie, Groupe Hospitalier Pitié-Salpétrière, 47 Boulevard de l’Hopital, F 75651 Paris Cedex 13, France

^aauthor for correspondence: fax 33-01-42-16-20-33, e-mail francoise.bismut@psl.ap-hop-paris.fr

Sensitive and accurate measurement of hepatic iron concentration (HIC) is required to investigate liver fibrogenesis (1) and its influence on the outcome of interferon therapy for chronic viral hepatitis C (2)(3). Hepatic iron content can be measured by a quantitative chemical method and/or evaluated by semiquantitative histologic scoring. Quantitative chemical methods assess all liver iron forms, whereas histologic scoring evaluates only the hemosiderin form. A colorimetric method using bathophenanthroline sulfonate as chromogen was recommended in 1978 by the International Committee for Standardization in Hematology (ICSH) for determination of serum iron (4)(5). It was adapted by Barry and Sherlock (6) to the determination of HIC, and we recently evaluated it for measurement of low HIC (7). In 1990, the ICSH replaced bathophenanthroline sulfonate with ferene, a more sensitive chromogen, in the determination of serum iron (8). The aim of the present study was to evaluate the replacement of bathophenanthroline sulfonate with ferene to improve the sensitivity of the colorimetric determination of low HIC.

We used samples of a frozen Wistar rat liver for quality control and determination of reliability criteria of both assays. We compared the results obtained with the two chromogens on 66 liver biopsies from patients with chronic liver diseases hospitalized in the Department of Hepatogastroenterology of the Pitié-Salpétrière Hospital. The clinical diagnoses of these patients are summarized in Table 1 . We determined the CV for HIC measurements on two separate samples from the same liver specimen for each chromogen on 38 human liver biopsies. Histologic iron scoring was according to the method of Deugnier et al. (9); among the 66 biopsies, 20 had no stainable iron (score of 0), and the 46 others exhibited iron overload (score 6). Biopsies . . . [Full Text of this Article]

References

The following articles in journals at HighWire Press have cited this article:

				E. Tolosano, S. Fagoonee, C. Garuti, L. Valli, N. C. Andrews, F. Altruda, and A. Pietrangelo Haptoglobin modifies the hemochromatosis phenotype in mice Blood, April 15, 2005; 105(8): 3353 - 3355. [Abstract] [Full Text] [PDF]

				S. Fagoonee, J. Gburek, E. Hirsch, S. Marro, S. K. Moestrup, J. M. Laurberg, E. I. Christensen, L. Silengo, F. Altruda, and E. Tolosano Plasma Protein Haptoglobin Modulates Renal Iron Loading Am. J. Pathol., April 1, 2005; 166(4): 973 - 983. [Abstract] [Full Text] [PDF]