HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) A correction has been published Submit an electronic Letter to the Editor about this paper Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Wu, H. Articles by Aboleneen, H. Search for Related Content PubMed PubMed Citation Articles by Wu, H. Articles by Aboleneen, H. Related Collections Endocrinology and Metabolism

Serum Estradiol Quantified by Isotope Dilution–Gas Chromatography/Mass Spectrometry

¹ Abbott Laboratories, Diagnostics Division, 100 Abbott Park Rd., Abbott Park, IL 60064

^aaddress correspondence to this author at: Abbott Laboratories, Department 90T, Bldg. AP20, 100 Abbott Park Rd., Abbott Park, IL 60064-3500; fax 847-938-7550, e-mail Hoda.aboleneen@abbott.com

Estradiol measurements are important in evaluations of ovarian function, infertility, and menopause (1). The assays are challenging because physiologic concentrations of estradiol are typically <100 ng/L in plasma of adult men and postmenopausal women and in both sexes during infancy and childhood (1). Immunoassays are widely used, and they provide high sensitivity and short analysis times (2). Other methods are needed to determine the accuracy of immunoassays. Our aim was to develop a practical gas chromatography–mass spectrometry (GC/MS) method to quantify serum estradiol and use it to evaluate immunoassay accuracy.

Isotope dilution–GC/MS is widely regarded as the most accurate technique for organic analytes of interest in clinical chemistry (3). Several GC/MS methods have been reported for the quantification of estradiol in biological sources (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). The complexity of samples requires isolation and purification of estradiol before derivatization for GC/MS analysis.

The most common separation methods for estradiol involve solvent extraction followed by further purification with Sephadex LH-20 (4)(5), strong ionic exchangers (6)(7)(8)(9), weak ionic exchanger (9)(10)(11), HPLC (12)(13), affinity chromatography (14), or their combinations. These methods, however, have limitations. The HPLC method for estradiol isolation cannot handle multiple samples simultaneously. The affinity column (14) is not commercially available and is very expensive to make. Other methods generally cannot sufficiently remove serum matrix materials to ensure rugged performance.

Here we report a GC/MS method for the quantification of serum estradiol in which estradiol is separated from the ether extract of serum samples by fractionation with a . . . [Full Text of this Article]

Acknowledgments

References

The following articles in journals at HighWire Press have cited this article:

				R. E. Nelson, S. K. Grebe, D. J. O'Kane, and R. J. Singh Liquid Chromatography-Tandem Mass Spectrometry Assay for Simultaneous Measurement of Estradiol and Estrone in Human Plasma Clin. Chem., February 1, 2004; 50(2): 373 - 384. [Abstract] [Full Text] [PDF]

				Z. Cao, T. A. Swift, C. A. West, T. G. Rosano, and R. Rej Immunoassay of Estradiol: Unanticipated Suppression by Unconjugated Estriol Clin. Chem., January 1, 2004; 50(1): 160 - 165. [Abstract] [Full Text] [PDF]