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Technical Briefs |
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University of Utah, Department of Pathology, Salt Lake City, UT 84132
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University of Mississippi Medical Center, Department of Pathology, Jackson, MS 39216
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Helena Laboratories, Beaumont, TX 77707
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Unilab Corporation, Tarzana, CA 91356
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Mayo Clinic, Department of Laboratory Medicine and Pathology, Rochester, MN 55905
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St. Bartholomew’s Hospital, Clinical Biochemistry Department, London EC1A 7BE, United Kingdom
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Reno Veterans Affairs Medical Center, Department of Pathology and Clinical Laboratory Services, Reno, NV 89520
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University of Missouri-Columbia School of Medicine, Departments of Child Health and Pathology, Columbia, MO 65212
aaddress correspondence to this author at: ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108; fax 801-584-5207, e-mail william.roberts@aruplab.com
Glycohemoglobin (gHb) is a biochemical marker of long-term glycemic control that is highly correlated with complications of diabetes mellitus (1). The presence of hemoglobin (Hb) variants can adversely affect the accuracy of some gHb methods, depending on the variant [for recent reviews, see Refs. (2)(3)]. Previous studies have shown that some gHb methods yield inaccurate results with samples heterozygous for Hb C or S (4)(5)(6)(7). In 2000, there were an estimated 26 million black or African Americans over age 17, of whom at least 10% had either Hb C or S trait (8)(9)(10)(11). The prevalence of diabetes is estimated to be 5.1% of the adult population, with rates for non-Hispanic blacks 1.6 times greater than in non-Hispanic whites (12). It is therefore probable that at least 200 000 Americans with diabetes have either Hb C or S trait. We investigated the measurement of gHb in specimens containing Hb C or S trait, using eight commercial gHb methods that are currently in clinical use.
Whole blood samples from individuals homozygous for Hb A (n = 43) and heterozygous for Hb C or S (n = 43 and 61, respectively) were collected in EDTA tubes. Hb variants were identified by their characteristic chromatograms obtained with a VARIANT analyzer (Bio-Rad Laboratories) operated according to the manufacturer’s instructions and using a Beta Thal Short program. Aliquots of these samples, containing 4–14% Hb A1c, were stored at 2–8 °C and analyzed within 10 days of collection. This study was approved by the Institutional Review Board of the University of Utah Health Sciences Center.
The CLC 330 gHb analyzer (Primus Corporation) was operated at the University of Missouri. This method was
Acknowledgments
References
The following articles in journals at HighWire Press have cited this article:
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  R. R. Little, C. L. Rohlfing, S. Hanson, S. Connolly, T. Higgins, C. W. Weykamp, M. D'Costa, V. Luzzi, W. E. Owen, and W. L. Roberts Effects of Hemoglobin (Hb) E and HbD Traits on Measurements of Glycated Hb (HbA1c) by 23 Methods Clin. Chem., August 1, 2008; 54(8): 1277 - 1282. [Abstract] [Full Text] [PDF]
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 J. M. Abadie and A. A. Koelsch Performance of the Roche Second Generation Hemoglobin A1c Immunoassay in the Presence of Hb-S or Hb-C Traits Ann. Clin. Lab. Sci., January 1, 2008; 38(1): 31 - 36. [Abstract] [Full Text] [PDF]
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S.-T. Lee, C. W. Weykamp, Y.-W. Lee, J.-W. Kim, and C.-S. Ki Effects of 7 Hemoglobin Variants on the Measurement of Glycohemoglobin by 14 Analytical Methods Clin. Chem., December 1, 2007; 53(12): 2202 - 2205. [Abstract] [Full Text] [PDF]
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Y. Wang, B. Beckwith, C. Smith, and G. Horowitz Misleading Glycated Hemoglobin Results in a Patient with Hemoglobin SC Disease Clin. Chem., July 1, 2007; 53(7): 1394 - 1395. [Full Text] [PDF]
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L. B. Thomas, S. J. Agosti, M. A. Man, and S. M. Mastorides Screening for Hemoglobinopathies During Routine Hemoglobin A1c Testing Using the Tosoh G7 Glycohemoglobin Analyzer Ann. Clin. Lab. Sci., January 1, 2007; 37(3): 251 - 255. [Abstract] [Full Text] [PDF]
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W. L. Roberts, S. Safar-Pour, B. K. De, C. L. Rohlfing, C. W. Weykamp, and R. R. Little Effects of Hemoglobin C and S Traits on Glycohemoglobin Measurements by Eleven Methods Clin. Chem., April 1, 2005; 51(4): 776 - 778. [Full Text] [PDF]
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R. R. Little, H. Vesper, C. L. Rohlfing, M. Ospina, S. Safar-Pour, and W. L. Roberts Validation by a Mass Spectrometric Reference Method of Use of Boronate Affinity Chromatography to Measure Glycohemoglobin in the Presence of Hemoglobin S and C Traits Clin. Chem., January 1, 2005; 51(1): 264 - 265. [Full Text] [PDF]
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 J L Camargo and J L Gross Conditions associated with very low values of glycohaemoglobin measured by an HPLC method J. Clin. Pathol., April 1, 2004; 57(4): 346 - 349. [Abstract] [Full Text] [PDF]
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