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Clinical Chemistry 48: 679-680, 2002;
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(Clinical Chemistry. 2002;48:679-680.)
© 2002 American Association for Clinical Chemistry, Inc.

Letters

Automated Assay for Fetal DNA Analysis in Maternal Serum

Jean-Marc Costa1a and Pauline Ernault1

1 M. Dassault Molecular Biology Laboratory, Centre de Diagnostic Prénatal, American Hospital of Paris, 63 bd Victor Hugo, 92200 Neuilly-sur-Seine, France

aAuthor for correspondence. E-mail jean-marc.costa@ahparis.org.


To the Editor:

Since the first demonstration of the presence of cell-free fetal DNA in maternal plasma of pregnant women (1), many reports have been published, mainly about fetal sex and RhD determinations (2)(3). Although good results have been obtained with conventional PCR, real-time PCR is now the most widely used amplification system for fetal DNA analysis because it allows high sensitivity with a high degree of protection against contamination (4). Conventional PCR may produce false-positive results from contamination (5), whereas real-time PCR, because it is a closed-tube system, reduces the risk of false-positive results from carryover of PCR products (6)(7). During sample preparation, however, contamination can result either from cross-contamination between samples or from the operator, particularly if a very small amount of human target sequence has to be detected, such as cell-free fetal DNA in . . . [Full Text of this Article]


Acknowledgments


References




The following articles in journals at HighWire Press have cited this article:


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R. W.K. Chiu, W.-b. Lui, A. El-Sheikhah, A. T.C. Chan, T. K. Lau, K. H. Nicolaides, and Y.M. D. Lo
Comparison of Protocols for Extracting Circulating DNA and RNA from Maternal Plasma
Clin. Chem., November 1, 2005; 51(11): 2209 - 2210.
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 J. Histochem. Cytochem.Home page
Y.M. D. Lo
Recent Advances in Fetal Nucleic Acids in Maternal Plasma
J. Histochem. Cytochem., March 1, 2005; 53(3): 293 - 296.
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J. Guibert, A. Benachi, A.-G. Grebille, P. Ernault, J.-R. Zorn, and J.-M. Costa
Kinetics of SRY gene appearance in maternal serum: detection by real time PCR in early pregnancy after assisted reproductive technique
Hum. Reprod., August 1, 2003; 18(8): 1733 - 1736.
[Abstract] [Full Text] [PDF]

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P. J. Johnson and Y.M. D. Lo
Plasma Nucleic Acids in the Diagnosis and Management of Malignant Disease
Clin. Chem., August 1, 2002; 48(8): 1186 - 1193.
[Abstract] [Full Text] [PDF]


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