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Clinical Chemistry 48: 960-963, 2002;
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(Clinical Chemistry. 2002;48:960-963.)
© 2002 American Association for Clinical Chemistry, Inc.

Technical Briefs

Comparative Evaluation of Serologic Tests for Celiac Disease Diagnosis and Follow-Up

Silvia Martini1, Giulio Mengozzi2a, Giuseppe Aimo2, Laura Giorda2, Roberto Pagni2 and Carla Sategna Guidetti1

1 Dipartimento di Medicina Interna, Università di Torino, 10126 Torino, Italy

2 UOA Laboratorio Analisi Chimico-Cliniche, Azienda Ospedaliera San Giovanni Battista, 10126 Torino, Italy

aauthor for correspondence

Anti-endomysium antibody (EmA) testing is used in the diagnosis of celiac disease (CD). The identification (1) of tissue transglutaminase (tTG) as the main antigen of EmA led to the development of commercial ELISAs for serum anti-tTG detection. At first, a guinea pig antigen (2)(3)(4)(5)(6)(7) yielded both sensitivity and specificity lower than those of EmA; therefore, human recombinant tTG (8)(9)(10)(11) was introduced to improve diagnostic accuracy and to overcome problems such as species specificity and cross-reactivity to contaminant proteins. However, the standardization of assays (12), the choice of cutoff value, the clinical relevance of these autoantibodies (13)(14), and the diagnostic accuracy of different commercial tests remain unresolved (15)(16)(17)(18). This study aimed to assess the diagnostic accuracy of five commercially available IgA anti-tTG ELISA reagent sets (four using a human recombinant and one a guinea-pig tTG antigen) for pathologically confirmed CD and to evaluate the changes in anti-tTG autoantibody concentrations during treatment of CD with a gluten-free diet (GFD).

This prospective study included 101 consecutive untreated adults (79 women and 22 men; median age, 37 years; range, 21–72 years) referred to University gastroenterologic outpatient clinic between January 2000 and May 2001 in whom CD was subsequently diagnosed by means of the typical appearance of small intestinal mucosa (19) (Marsh grade III in 95 patients and grade II in 6 patients) and by a positive clinical response to a GFD. We reported on 34 of these patients (all EmA-positive) previously (17). A duodenal biopsy was performed in all patients on the basis of clinical history and serologic assessment, including EmA testing and nutritional . . . [Full Text of this Article]


References




The following articles in journals at HighWire Press have cited this article:


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 CVIHome page
E. Zintzaras and A. E. Germenis
Performance of Antibodies against Tissue Transglutaminase for the Diagnosis of Celiac Disease: Meta-Analysis
Clin. Vaccine Immunol., February 1, 2006; 13(2): 187 - 192.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
B. Van Meensel, M. Hiele, I. Hoffman, S. Vermeire, P. Rutgeerts, K. Geboes, and X. Bossuyt
Diagnostic Accuracy of Ten Second-Generation (Human) Tissue Transglutaminase Antibody Assays in Celiac Disease
Clin. Chem., November 1, 2004; 50(11): 2125 - 2135.
[Abstract] [Full Text] [PDF]

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 Arch Pediatr Adolesc MedHome page
J.-J. Baudon, C. Johanet, Y. B. Absalon, G. Morgant, S. Cabrol, and J.-F. Mougenot
Diagnosing Celiac Disease: A Comparison of Human Tissue Transglutaminase Antibodies With Antigliadin and Antiendomysium Antibodies
Arch Pediatr Adolesc Med, June 1, 2004; 158(6): 584 - 588.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M J. Llorente, M. Sebastian, M J. Fernandez-Acenero, G. Prieto, and S. Villanueva
IgA Antibodies against Tissue Transglutaminase in the Diagnosis of Celiac Disease: Concordance with Intestinal Biopsy in Children and Adults
Clin. Chem., February 1, 2004; 50(2): 451 - 453.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
D. Basso, G. Guariso, and M. Plebani
Serologic Testing for Celiac Disease
Clin. Chem., November 1, 2002; 48(11): 2082 - 2083.
[Full Text] [PDF]


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