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Direct Spectrophotometric Method for Measurement of Bilirubin in Newborns: Comparison with HPLC and an Automated Diazo Method

¹ Department of Pathology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Rd., Portland, OR 97201; Departments of
² Pediatrics and
³ Pathology and Laboratory Medicine, Brody School of Medicine of East Carolina University, Greenville, NC 27858;
⁴ Department of Pediatrics and Waisman Center, University of Wisconsin, 1500 Highland Ave., Madison, WI 53705

^aaddress correspondence to this author at: Department of Pathology, Oregon Health and Sciences University, Mailcode L471, 3181 SW Sam Jackson Park Rd., Portland, OR 97201; fax 503-494-8148, e-mail kazmierc@ohsu.edu

The first 20% of the full text of this article appears below.

The measurement of serum total bilirubin is one of the most frequently performed tests in newborns (1)(2)(3). Direct spectrophotometry for the measurement of bilirubin in sera from newborns is a simple and rapid method that requires minimal sample for analysis (4). The direct spectrophotometric assay is based on the absorbance of bilirubin at 454 nm; by contrast, hemoglobin absorbs equally at both 454 and 528 nm. Subtraction of the absorbance at 528 nm from that at 454 nm eliminates the effect of hemolysis and yields a value that can be attributed primarily to bilirubin. Unfortunately, other pigments, such as carotenoids, also absorb at 454 nm, thus limiting this technique to neonates <2–3 weeks of age.

Direct spectrophotometric methods have been compared with diazo (Jendrassik–Gróf-based) bilirubin methods (5)(6)(7)(8)(9), but sample hemolysis can increase or decrease the results obtained by the latter procedure, depending on the concentrations of hemoglobin and bilirubin in the sample (10)(11). Most such studies either do not describe the effect of sample hemolysis on the agreement between methods or report poorer agreement between methods or increased scatter around the regression line (6)(8)(9).

HPLC for measurement of serum bilirubin concentrations is labor-intensive and . . . [Full Text of this Article]

The following articles in journals at HighWire Press have cited this article:

				J. M Kirk Neonatal jaundice: a critical review of the role and practice of bilirubin analysis Ann Clin Biochem, September 1, 2008; 45(5): 452 - 462. [Abstract] [Full Text] [PDF]

				D De Luca, E Zecca, M Corsello, E Tiberi, C Semeraro, and C Romagnoli Attempt to improve transcutaneous bilirubinometry: a double-blind study of Medick BiliMed versus Respironics BiliCheck Arch. Dis. Child. Fetal Neonatal Ed., March 1, 2008; 93(2): F135 - F139. [Abstract] [Full Text] [PDF]