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Clinical Chemistry 48: 1114-1116, 2002;
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Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 2002;48:1114-1116.)
© 2002 American Association for Clinical Chemistry, Inc.

Technical Briefs

Preanalytical Influences on the Measurement of Ghrelin

Michael Gröschl1a, Roland Wagner1, Jörg Dötsch1, Wolfgang Rascher1 and Manfred Rauh1

1 Kinderklinik Erlangen, Loschgestrasse 15, 91054 Erlangen, Germany

aauthor for correspondence: fax 0049-09131-8533745, e-mail michael.groeschl@kinder.imed.uni-erlangen.de

The first 20% of the full text of this article appears below.

Ghrelin is an acylated peptide with growth-hormone- releasing function (1)(2). It was first isolated from rat stomach during the search for an endogenous ligand to an "orphan" G-protein-coupled receptor (3). The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is indispensable for biological activity. Human ghrelin differs from rat ghrelin by only two amino acids at positions 11 and 12. The peptide stimulates the release of growth hormone when administered intravenously to rats and given to rat primary pituitary cells (2).

In previous studies, serum was preferred for the determination of ghrelin. Experience with other sample materials obtained after administration of various anticoagulating substances has not yet been described. It is therefore unknown which method of obtaining samples for ghrelin determination enables the most accurate and precise measurements. Furthermore, data on the stability of the hormone are still lacking, but are necessary for optimizing analytical conditions.

The objective of the present study was to compare the reliability of ghrelin measurements in serum and four different plasma samples and to evaluate data on stability under different storage conditions.

Blood samples were taken from apparently healthy volunteers (10 men and 4 women; age range, 18–40 years) who were not on medication and had normal blood pressure. The body mass index varied from 20 to 29 kg/m2. Blood was taken between 1000 and 1100 by venipuncture (Multifly® with 20-mL cannulas; Sarstedt) and immediately divided into tubes for plasma preparation with dipotassium EDTA (Kabe), citrate, fluoride, and lithium heparinate (Sarstedt) as anticoagulating substances. The content of liquid anticoagulating additive in citrate-plasma tubes was 118 ± 15 µL (n = 15; mean ± SD). Additionally, serum was prepared from each sample (Sarstedt). After clotting, samples were centrifuged . . . [Full Text of this Article]




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