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Clinical Chemistry 49: 1019-1021, 2003; 10.1373/49.6.1019
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(Clinical Chemistry. 2003;49:1019-1021.)
© 2003 American Association for Clinical Chemistry, Inc.

Technical Briefs

Use of Inductively Coupled Plasma Mass Spectrometry to Measure Urinary Iodine in NHANES 2000: Comparison with Previous Method

Kathleen L. Caldwell1,a, C. Brook Maxwell1, Amir Makhmudov1, Sam Pino2, Lewis E. Braverman2, Robert L. Jones1 and Joseph G. Hollowell3

1 Inorganic Toxicology and Nutrition Branch, Division of Laboratory Sciences, National Center for Environmental Health, CDC, 4770 Buford Hwy., NE, Mail Stop F18, Atlanta, GA 30341

2 Section of Endocrinology, Diabetes, and Nutrition, Department of Medicine, Boston Medical Center, Evans Bldg., Room 201, 88 Newton St., Boston, MA 02118-2393

3 Department of Pediatrics, University of Kansas Medical Center, 435 N. 1500 Road, Lawrence, KS 66049

aauthor for correspondence: fax 770-488-4609, e-mail Klc7@cdc.gov

The first 20% of the full text of this article appears below.

Urinary iodine (UI) concentrations directly reflect dietary iodine intake and consequently test biochemical assessment of the iodine status worldwide (1).

The Iodine Laboratory of the Division of Laboratory Sciences at the National Center for Environmental Health, CDC, measured the UI content of specimens as part of the National Health and Nutrition Examination Survey (NHANES) 2000 and will measure UI in the US population through future NHANES analyses, using inductively coupled plasma mass spectrometry (ICP-MS). In this report, we describe the ICP-MS laboratory method and compare that method with the established Sandell–Kolthoff (S-K) spectrophotometric method used in NHANES III.

The ICP-MS method described previously (2) was modified for use in NHANES 2000 by adding alkaline diluents and rinses to measure UI as follows: urine samples and the urine iodide calibrator solutions were prepared just before analysis by dilution (1:49; 50 µL of sample/calibrator plus 2450 µL of diluent) with an aqueous solution of 10 mL/L tetramethylammonium hydroxide containing 10 µg/L tellurium as an internal standard. A peristaltic pump introduced the diluted samples into the spray chamber with an argon stream. The I+ and Te+ ions were measured at m/z 127 and 130, respectively (3). The diluent used to make the intermediate working calibrators was 1.0 g of analytical-reagent-grade sodium thiosulfate (Na2S2O3) dissolved in 1000 mL of 18 M{Omega} · cm ultrapure water. (The water used for all dilutions and rinses in the ICP-MS method is ultrapure 18 M{Omega} · cm water; Millipore Corporation.) The wash solution was an aqueous solution of 1 mL/L Triton X-100 and 10 . . . [Full Text of this Article]






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