Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 49: 979-981, 2003; 10.1373/49.6.979
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Karlsson, H.
Right arrow Articles by Kristenssson, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karlsson, H.
Right arrow Articles by Kristenssson, K.
Related Collections
Right arrow Molecular Diagnostics and Genetics
Right arrow Other Areas of Clinical Chemistry
(Clinical Chemistry. 2003;49:979-981.)
© 2003 American Association for Clinical Chemistry, Inc.

Technical Briefs

Extraction of RNA from Dried Blood on Filter Papers after Long-Term Storage

Håkan Karlsson1,a, Claes Guthenberg2, Ulrika von Döbeln2 and Krister Kristenssson1

1 Division of Neurodegenerative Diseases, Department of Neuroscience, Karolinska Institutet, S-171 77 Stockholm, Sweden

2 PKU Laboratory, Center for Inherited Metabolic Diseases, Huddinge University Hospital, 141 86 Huddinge, Sweden

aaddress correspondence to this author at: Department of Neuroscience, Karolinska Institutet, Retzius väg 8, S-171 77 Stockholm, Sweden; fax 46-8-32-53-25, e-mail hakan.karlsson@neuro.ki.se

The first 20% of the full text of this article appears below.

Blood dried on filter paper is widely used for screening of inherited metabolic disorders (1). In Sweden, such filters from all newborns have been permanently stored since 1975. It has been shown that proteins and DNA may be recovered from these cards after extended periods of storage (2)(3)(4). RNA, however, has been considered too vulnerable to degradation by ribonucleases to be recovered from these filters. Despite this, Zhang and McCabe (5) and Matsubara et al.(6) reported that mRNA could be isolated from such filters after up to 4 years of storage. The stability of viral RNA on filters has also been reported (7)(8), although this was not tested over extended periods of time. The purpose of the present study was to investigate whether RNA could be recovered from filters that had been stored since 1975 and be amplified by reverse transcription-PCR.

After approval by the local ethics committee, we randomly selected filter papers (specimen collection paper 2992; Schleicher & Schuell) that had been stored for 1 month, 21 years, and 27 years; for each time point, we selected five filters. One-fourth of a spot (~0.3 cm2) containing dried blood was cut out . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Med. Genet.Home page
U Hannelius, C M Lindgren, E Melen, A Malmberg, U von Dobeln, and J Kere
Phenylketonuria screening registry as a resource for population genetic studies
J. Med. Genet., October 1, 2005; 42(10): e60 - e60.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Association for Clinical Chemistry.