Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 50: 231-233, 2004; 10.1373/clinchem.2003.025536
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chim, S. S.C.
Right arrow Articles by Lo, Y.M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chim, S. S.C.
Right arrow Articles by Lo, Y.M. D.
Related Collections
Right arrow Molecular Diagnostics and Genetics
(Clinical Chemistry. 2004;50:231-233.)
© 2004 American Association for Clinical Chemistry, Inc.

Technical Briefs

Genomic Sequencing of a SARS Coronavirus Isolate That Predated the Metropole Hotel Case Cluster in Hong Kong

Stephen S.C. Chim1, Yu-Kwan Tong1, Emily C.W. Hung2, Rossa W.K. Chiu1 and Y.M. Dennis Lo1,a

Departments of
1 Chemical Pathology and
2 Paediatrics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong

aaddress correspondence to this author at: Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Room 38023, 1/F Clinical Sciences Bldg., 30-32 Ngan Shing St., Shatin, New Territories, Hong Kong SAR; fax 852-2194-6171, e-mail loym@cuhk.edu.hk

The first 20% of the full text of this article appears below.

The epidemic of severe acute respiratory syndrome (SARS) swept across the globe, with reported cases in more than 30 countries. As of July 11, 2003, the number of reported probable cases was 8437, with 813 deaths (1). A novel coronavirus, SARS-CoV, was promptly implicated as the causative agent (2)(3)(4). Macaques infected with SARS-CoV subsequently developed respiratory symptoms and pathology similar to SARS patients, thus fulfilling the Koch postulates (5). Efforts in sequencing the viral genome promptly followed, and the genomic sequence revealed little homology to previously characterized strains of coronaviruses (6)(7). The complete genomic sequences of several SARS-CoV isolates have since been made publicly available (www.ncbi.nlm.nih.gov).

Several sequence variations exist among isolates. In general, based on these sequence variations, the majority of the isolates can be segregated into two groups: isolates that were obtained from individuals who were epidemiologically linked to and those who were not linked to the Metropole Hotel in Hong Kong (8)(9)(10). Ruan et al. (10) compared the genomic sequences of 14 SARS-CoV isolates and suggested that a haplotype comprising four nucleotide positions, namely, 9404, 17564, 22222, and 27827 [GenBank accession no. AY274119 (7)], clearly defined two distinct genotypes. Isolates that were epidemiologically linked to the Metropole Hotel cluster have the configuration T:T:T:T, as opposed to the sequence C:G:C:C seen in the unassociated strains. (Note: The usage of the DNA-based code for the designation of SARS-CoV haplotypes does not imply that this virus possesses a DNA genome.)

SARS was first reported in Guangdong Province, China, in November 2002 (11). Isolates that demonstrated the C:G:C:C haplotype were epidemiologically . . . [Full Text of this Article]






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Association for Clinical Chemistry.