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Immunoluminometric Assay for the Midregion of Pro-Atrial Natriuretic Peptide in Human Plasma

¹ Research Department, B.R.A.H.M.S AG, Biotechnology Centre Hennigsdorf/Berlin, 16761 Hennigsdorf, Germany

^aauthor for correspondence: fax 49-3302-883-451, e-mail n.morgenthaler@brahms.de

The first 20% of the full text of this article appears below.

The prohormone of atrial natriuretic peptide (proANP) is a polypeptide of 126 amino acids. Mature atrial natriuretic peptide (ANP) consists of amino acids 99–126 and comprises 98% of the natriuretic peptides in the circulation (1). The N-terminal portion of proANP, termed proANP1–98 or NT-proANP, has a much longer half-life than mature ANP and has therefore been suggested to be a more reliable analyte for measurement than mature ANP (2). In addition to other established indications, proANP has recently gained much interest as a potential new marker in the field of sepsis (3), emphasizing the need for a reliable assay for this molecule.

All sandwich immunoassays developed for proANP to date use an antibody against the N-terminal region of proANP1–98 combined with a second antibody against either the midregion (4) or C-terminal region (5)(6). However, under certain conditions, the N-terminal region might be minimally accessible for antibody binding (7)(8). Despite the described long half-life of proANP, results from various competitive immunoassays as well as HPLC analyses indicate that proANP1–98 can be subject to further fragmentation (9)(10).

We developed a new sandwich immunoassay for midregional proANP (amino acids 53–90; Fig. 1A ). With this assay, which was named B.R.A.H.M.S SERISTRA® (B.R.A.H.M.S AG), we measured proANP values in 325 healthy controls.

View larger version (19K): [in this window] [in a new window]   Figure 1. Representation of the assay principle for midregional proANP (A), and interassay imprecision for 25 plasma samples measured on 10 different days (B). (A), numbers indicate amino acids (aa). (B), the interassay CV . . . [Full Text of this Article]

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