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Clinical Chemistry 50: 256-257, 2004; 10.1373/clinchem.2003.026013
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(Clinical Chemistry. 2004;50:256-257.)
© 2004 American Association for Clinical Chemistry, Inc.

Letters

EDTA Is a Better Anticoagulant than Heparin or Citrate for Delayed Blood Processing for Plasma DNA Analysis

Nicole Y.L. Lam1, Timothy H. Rainer1, Rossa W.K. Chiu2 and Y.M. Dennis Lo2,a

1 A&E Medicine Academic Unit and
2 Department of Chemical Pathology The Chinese University of Hong Kong Prince of Wales Hospital Shatin, New Territories, Hong Kong SAR

aAuthor for correspondence. Fax 852-2194-6171; e-mail loym@cuhk.edu.hk.

The first 20% of the full text of this article appears below.


To the Editor:

Plasma is usually chosen for cell-free circulating DNA studies because ex vivo release of DNA from hematopoietic cells was demonstrated in serum during the clotting process from whole blood (1)(2)(3). Among the most commonly used anticoagulants, EDTA, heparin, and citrate, heparin was suggested by some investigators to be an unsuitable anticoagulant because it could inhibit PCR (4)(5)(6). However, the quantitative effects of the choice of anticoagulants for plasma DNA analysis have not been studied systematically. We therefore conducted an experiment to investigate the effect of three commonly used anticoagulants on plasma DNA quantification.

Ten healthy individuals were recruited, and peripheral blood was collected into four EDTA, four heparin, and four citrate tubes (Vacuette; Greiner) for each individual. Blood samples were kept at room temperature (24°C). Plasma was obtained by centrifugation from the EDTA, heparin, and citrate tubes at four different . . . [Full Text of this Article]




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