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Technical Briefs |
1 Department of Histology, Medical Embryology, Obstetrics and Gynecology, University of Bologna, Bologna, Italy;
2 Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China;
aaddress correspondence to this author at: Department of Histology, Medical Embryology, Obstetrics and Gynecology, University of Bologna, Policlinico S. Orsola Malpighi, Bologna, Italy; fax 39-051-2094110, e-mail antonio.farina@unibo.it
| The first 20% of the full text of this article appears below. |
Higher concentrations of circulating nucleic acids (DNA and RNA) in maternal plasma/serum have been reported in pregnancies complicated by preeclampsia (PE) compared with controls matched for gestational age (1)(2)(3)(4)(5). Fetal and total DNA concentrations were also demonstrated to be directly correlated to both gestational age and to the severity of PE (6). Again, both hypertension and proteinuria (the two main symptoms for PE classification) are independently associated with an increase in circulating fetal DNA (6). Most studies, however, have had to rely on Y-chromosome loci, which can be used as fetus-specific markers for male fetuses. For this reason, the identification in maternal plasma of fetal-gender-independent markers, such as mRNA transcripts from genes expressed in fetal placenta, represented an important development in the field (4). The concentrations of mRNA for corticotropin-releasing hormone (CRH) in maternal plasma are significantly higher in PE than in controls (5), but no demonstration of relationships with gestational age and/or severity of PE have been reported.
The aim of this study was to evaluate and quantify the relationships of the concentration of circulating CRH mRNA in maternal plasma with both gestational age and the severity of PE.
We conducted a retrospective study in which the control group was 17 women with uncomplicated pregnancies (35% males) during the third trimester (median, 259 days since last menstrual period; minimum–maximum, 189–281 days). Controls were matched with 17 pregnancies (53% males) affected by PE at the time of blood collection (median, 256 days since last menstrual period; minimum–maximum, 181–279 days). PE was defined as gestational hypertension (systolic pressure >140 mmHg or diastolic blood pressure >90 mmHg on at
The following articles in journals at HighWire Press have cited this article:
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  S. Okazaki, A. Sekizawa, Y. Purwosunu, A. Farina, N. Wibowo, and T. Okai Placenta-Derived, Cellular Messenger RNA Expression in the Maternal Blood of Preeclamptic Women Obstet. Gynecol., November 1, 2007; 110(5): 1130 - 1136. [Abstract] [Full Text] [PDF]
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 Y. Purwosunu, A. Sekizawa, K. Koide, A. Farina, N. Wibowo, G. H. Wiknjosastro, S. Okazaki, H. Chiba, and T. Okai Cell-Free mRNA Concentrations of Plasminogen Activator Inhibitor-1 and Tissue-Type Plasminogen Activator Are Increased in the Plasma of Pregnant Women with Preeclampsia Clin. Chem., March 1, 2007; 53(3): 399 - 404. [Abstract] [Full Text] [PDF]
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 S Okazaki, A Sekizawa, Y Purwosunu, M Iwasaki, A Farina, and T Okai Measurement of mRNA of trophoblast-specific genes in cellular and plasma components of maternal blood. J. Med. Genet., September 1, 2006; 43(9): e47 - e47. [Abstract] [Full Text] [PDF]
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R. W.K. Chiu, W.-b. Lui, M.-c. Cheung, N. Kumta, A. Farina, I. Banzola, S. Grotti, N. Rizzo, C. J. Haines, and Y.M. D. Lo Time Profile of Appearance and Disappearance of Circulating Placenta-Derived mRNA in Maternal Plasma Clin. Chem., February 1, 2006; 52(2): 313 - 316. [Abstract] [Full Text] [PDF]
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X. Y. Zhong, S. Gebhardt, R. Hillermann, K. C. Tofa, W. Holzgreve, and S. Hahn Parallel Assessment of Circulatory Fetal DNA and Corticotropin-Releasing Hormone mRNA in Early- and Late-Onset Preeclampsia Clin. Chem., September 1, 2005; 51(9): 1730 - 1733. [Full Text] [PDF]
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H. Masuzaki, K. Miura, K. Yamasaki, S. Miura, K.-i. Yoshiura, S. Yoshimura, D. Nakayama, C. K. Mapendano, N. Niikawa, and T. Ishimaru Clinical Applications of Plasma Circulating mRNA Analysis in Cases of Gestational Trophoblastic Disease Clin. Chem., July 1, 2005; 51(7): 1261 - 1263. [Full Text] [PDF]
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H. Masuzaki, K. Miura, K.-i. Yoshiura, K. Yamasaki, S. Miura, S. Yoshimura, D. Nakayama, C. K. Mapendano, N. Niikawa, and T. Ishimaru Placental mRNA in Maternal Plasma and Its Clinical Application to the Evaluation of Placental Status in a Pregnant Woman with Placenta Previa-Percreta Clin. Chem., May 1, 2005; 51(5): 923 - 925. [Full Text] [PDF]
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