Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 50: 1942-1943, 2004; 10.1373/clinchem.2004.036830
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Campbell, B.
Right arrow Articles by El Shami, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campbell, B.
Right arrow Articles by El Shami, S.
Related Collections
Right arrow Molecular Diagnostics and Genetics
Right arrow Oak Ridge Conference
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 2004;50:1942-1943.)
© 2004 American Association for Clinical Chemistry, Inc.

Abstracts of Oak Ridge Posters

Nucleic Acid Testing Using Surface Plasmon Resonance Fluorescence Detection

Bruce Campbella, Jason Lei, David Kiaei, Dennis Sustarsic and Saïd El Shami

1 Diagnostic Products Corporation, 5700 West 96th St., Los Angeles, CA 90045

aauthor for correspondence: fax 310-645-9999, e-mail bcampbell@dpconline.com

The first 20% of the full text of this article appears below.

The completion of the Human Genome Project (1) is likely to accelerate the demand for nucleic acid diagnostics, one of the fastest growing segments of in vitro diagnostics. To meet this demand, fully automated, sensitive, and specific technologies for nucleic acid detection are needed.

Surface plasmon resonance fluorescence (SPRF) is an optical detection technology that offers kinetic analysis of biomolecular interactions in real time (2). SPRF technology uses a sensor composed of a dielectric material coated with a thin layer of a noble metal. An evanescent field is generated at the metal-dielectric interface through interaction of p-polarized light with surface plasmons (oscillating electrons) under a resonance condition. The evanescent field excites a fluorophore localized near the metal surface, which in turn emits evanescent waves, generating surface plasmons at the fluorescence emission frequency. These propagating waves (plasmon-mediated emission) radiate through the metal film and dielectric material. This emission emerges as a cone of radiation in the dielectric material.

Application of SPRF for nucleic acid testing involves immobilization of synthetic oligonucleotide capture probes on the sensor surface (Fig. 1 ). Biotin-tagged nucleic acid amplicons are hybridized to target-specific capture probes and detected with a streptavidin-fluorophore conjugate by monitoring of the plasmon-mediated emission over time. Because the evanescent field penetrates a short distance into the medium above the metal surface, the hybridized amplicons are selectively excited and detected. This feature of SPRF allows sensitive detection without a wash step to separate the free from the hybridized nucleic acids. The fluorescent emission is collected by a patented optical system, providing higher sensitivity than other . . . [Full Text of this Article]






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Association for Clinical Chemistry.