HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Levy, N. S. Articles by Levy, A. P. Search for Related Content PubMed PubMed Citation Articles by Levy, N. S. Articles by Levy, A. P. Related Collections Molecular Diagnostics and Genetics Proteomics and Protein Markers Lipids, Lipoproteins, and Cardiovascular Risk Factors

ELISA for Determination of the Haptoglobin Phenotype

¹ Technion Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel

^aaddress correspondence to this author at: Rappaport Medical Building, Technion-Israel Institute of Technology, Bat Galim Haifa, Israel 31096; fax 972-4-8514103, e-mail alevy@tx.technion.ac.il

The first 300 words of the full text of this article appear below.

The haptoglobin genetic locus at 16q22 is polymorphic with two known classes of alleles, denoted 1 and 2 (1). The polymorphism is extremely common, with worldwide frequencies of the two alleles being approximately equal. However, there is considerable geographic and ethnic variation in the distribution of haptoglobin phenotypes (1). Over the past 3–4 years our laboratory has demonstrated that haptoglobin is a major susceptibility gene for the development of diabetic vascular complications in multiple longitudinal and cross-sectional population studies (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). Diabetic individuals homozygous for the haptoglobin 2 allele were shown to be at five times greater risk of developing cardiovascular disease compared with diabetic individuals homozygous for the haptoglobin 1 allele, with an intermediate risk for the heterozygote (8). The increased susceptibility to vascular complications conferred by the Hp 2 allele has recently been recapitulated in a transgenic animal model, which showed direct linkage of the polymorphism with disease susceptibility (R. Lotan et al., manuscript submitted). Mechanistic studies using the purified protein products of the Hp 1 and Hp 2 alleles have identified profound differences in antioxidant and immunomodulatory activity (14)(15).

Functional as well as structural differences exist between the various haptoglobin allelic protein products (1). The Hp 2 allele appears to have arisen by an intragenic duplication event of exons 3 and 4 of the Hp 1 allele, which leads to the duplication of a multimerization domain in exon 3. Consequently, the Hp 1 allele protein product forms dimers only. The Hp 2 allele has two copies of exon 3; therefore, Hp 2 allele protein products combine to form cyclic polymers three monomers . . . [Full Text of this Article]

The following articles in journals at HighWire Press have cited this article:

				M. Soejima and Y. Koda TaqMan-Based Real-Time PCR for Genotyping Common Polymorphisms of Haptoglobin (HP1 and HP2) Clin. Chem., November 1, 2008; 54(11): 1908 - 1913. [Abstract] [Full Text] [PDF]

				J. R. Delanghe and M. L. De Buyzere Development of an ELISA for the Determination of the Major Haptoglobin Phenotype: An Interesting Technical Development and Its Potential Consequences Clin. Chem., November 1, 2004; 50(11): 1972 - 1973. [Full Text] [PDF]