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Comparison of Methods for Polycythemia Rubra Vera-1 mRNA Quantification in Whole-Blood Leukocytes and Purified Granulocytes

¹ Department of Clinical Chemistry and Transfusion Medicine, Institute of Laboratory Medicine and ² Hematology and Coagulation Section, Department of Medicine, Sahlgrenska University Hospital, Göteborg, Sweden³ Department of Medicine, Uddevalla Hospital, Uddevalla, Sweden⁴ Department of Experimental Anaesthesiology, University Hospital, Freiburg, Germany

^aaddress correspondence to this author at: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, SE-413 45 Göteborg, Sweden; fax 46-31-828458, e-mail anne.ricksten@clinchem.gu.se

The first 300 words of the full text of this article appear below.

In the absence of pathognomonic markers, the diagnosis of the two chronic myeloproliferative disorders polycythemia vera (PV) and essential thrombocythemia (ET) has relied on a set of clinical and laboratory criteria (1)(2)(3)(4)(5). The cloning of the cell surface receptor polycythemia rubra vera-1 (PRV-1) has recently been described (6), and the consistent overexpression of PRV-1 mRNA observed in PV patients indicates that this might constitute a new diagnostic marker for the disease. In the initial cohort examined by Northern blot analysis, PRV-1 expression was increased in all PV patients examined as well as in some ET patients, but not in healthy controls (6). These results have also been verified and extended using a quantitative reverse transcription-PCR (RT-PCR) assay. All PV as well as 50% of ET patients displayed increased PRV-1 expression (7)(8). Patients with secondary erythrocytosis and healthy controls tested showed PRV-1 concentrations within the reference interval. Interestingly, the observed increase in PRV-1 mRNA expression does not lead to a corresponding increase in protein expression on the cell surface (9). Erythropoietin-independent colony growth and PRV-1 overexpression seem to go hand in hand in both PV and ET patients (7), raising the hope that RT-PCR for PRV-1 could replace the need for the technically demanding erythropoietin-independent colony assay, although a recent report suggests that the erythropoietin-independent colony growth assay is a more reliable method (10). The aim of the present work was to develop a quantitative RT-PCR method to measure PRV-1 transcripts in whole-blood leukocytes and to determine the potential usefulness of the method in the differential diagnosis of polycythemias and thrombocytosis. The assay was compared with a method using isolated granulocytes (7)(9) to . . . [Full Text of this Article]

The following articles in journals at HighWire Press have cited this article:

				L. Teofili, M. Martini, F. Guidi, D. Venditti, G. Leone, and M. L. Larocca The PRV-1 gene expression in essential thrombocythemia Blood, November 1, 2004; 104(9): 2995 - 2996. [Full Text] [PDF]