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Clinical Chemistry 50: 1413-1414, 2004; 10.1373/clinchem.2004.032979
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(Clinical Chemistry. 2004;50:1413-1414.)
© 2004 American Association for Clinical Chemistry, Inc.

Technical Briefs

Detection of Placental Transcription Factor mRNA in Maternal Plasma

Attie T.J.I. Go1, Allerdien Visser2, Monique A.M. Mulders2, Marinus A. Blankenstein2, John M.G. van Vugt1 and Cees B.M. Oudejans2,a

Departments of1 Obstetrics and Gynaecology and2 Clinical Chemistry, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands

aauthor for correspondence: e-mail cbm.oudejans@vumc.nl

The first 20% of the full text of this article appears below.

mRNA of placental origin, including chromosome 21-encoded mRNA, can be detected reliably in maternal plasma during the first trimester of pregnancy (1)(2). The presence and detectability of placental RNA in maternal plasma permits rapid screening of new markers to test their feasibility for use in noninvasive prenatal diagnostic assays. In contrast to conventional protein-based assays, new markers can include gene products with intracellular localization and noncoding mRNA. We challenged these features by screening maternal plasma for a large number of RNA targets (n = 80) known or expected to be present in extraembryonic tissues. This set included genes coding for transcription factors, genes subject to genomic imprinting, genes coding for noncoding RNA, and other genes with restricted or abundant expression in trophoblast cells. Target genes were distributed over all chromosomes except the Y chromosome.

Peripheral blood samples were collected from pregnant women attending the Prenatal Diagnostic Centre of the VU University Medical Center. All participants gave informed consent before being included in the study. The study was approved by the VU University Medical Center Ethics Committee. EDTA blood was collected between weeks 9 and 13 of pregnancy. All blood samples were obtained before invasive diagnostic procedures and processed as described previously (2). RNA was extracted from 1.6 mL of maternal plasma by silica-based affinity isolation with use of the QIAamp MinElute Virus Vacuum system (Qiagen) with minor modifications (2). The amount of . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 Hum Reprod UpdateHome page
C. F. Wright and H. Burton
The use of cell-free fetal nucleic acids in maternal blood for non-invasive prenatal diagnosis
Hum. Reprod. Update, October 22, 2008; (2008) dmn047v1.
[Abstract] [Full Text] [PDF]


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