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Ki-67 Protein Concentrations in Urothelial Bladder Carcinomas Are Related to Ki-67-Specific RNA Concentrations in Urine

¹ Institut fuer Molekulare Medizin,² Institut fuer Pathologie, and³ Klinik und Poliklinik fuer Urologie, UK-SH, Campus Luebeck and Universitaet zu Luebeck, Luebeck, Germany;⁴ HELIOS Agnes Karll Krankenhaus, Bad Schwartau, Germany

^aaddress correspondence to this author at: Institut fuer Molekulare Medizin, Universitaet zu Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany; fax 49-451-500-2729, e-mail warnecke@imm.uni-luebeck.de

The first 300 words of the full text of this article appear below.

The Ki-67 protein is a nuclear and nucleolar protein that is strictly associated with cell proliferation. Recently it has been suggested to play a role in the control of the higher order chromatin structure (1). Because the protein is produced only in dividing cells, the anti Ki-67 antibody MIB-1 has been widely used in histopathologic studies to estimate the growth fraction of human neoplastic tissue samples in situ. For a variety of human tumors, including bladder carcinomas, the Ki-67 labeling index has been shown to be of prognostic value for tumor recurrence and for patient survival (2)(3)(4). We investigated whether Ki-67 RNA in total urine of bladder tumor patients is correlated with the Ki-67 labeling index of the corresponding tumor tissue.

Spontaneously voided clean-catch urines from 68 patients were collected with informed consent, stored at 4 °C, and processed within 4 h of collection. Approval was obtained from the Ethikkommission of the University of Luebeck. Three groups of patients were included: (a) healthy donors (n = 14); (b) patients with urinary tract infection as detected by analysis of urine sediment (n = 28); and (c) patients with bladder carcinoma (n = 26). All urine samples from patients with tumors were checked for significant bacteriuria or leukocyturia (>10/µL) as well as for the presence of erythrocytes (>10/µL). The specific weights of all urine samples were in the range 1.010–1.015 kg/L, showing that there were no major differences in urine concentration. After routine diagnostics were completed, 1 mL of urine was mixed 1:1 with lysis buffer [5.64 mol/L guanidinium thiocyanate, 5 g/L sarcosyl, 50 mmol/L sodium acetate (pH 6.5), 1 mmol/L ß-mercaptoethanol], the pH was adjusted to 7 by addition of 1.5 mol/L HEPES (pH 8.0), and samples . . . [Full Text of this Article]

The following articles in journals at HighWire Press have cited this article:

				M. Hanke, I. Kausch, G. Dahmen, D. Jocham, and J. M. Warnecke Detailed Technical Analysis of Urine RNA-Based Tumor Diagnostics Reveals ETS2/Urokinase Plasminogen Activator to Be a Novel Marker for Bladder Cancer Clin. Chem., December 1, 2007; 53(12): 2070 - 2077. [Abstract] [Full Text] [PDF]