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Clinical Chemistry 50: 1673-1674, 2004; 10.1373/clinchem.2004.033548
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(Clinical Chemistry. 2004;50:1673-1674.)
© 2004 American Association for Clinical Chemistry, Inc.

Technical Briefs

Detection of Staphylococcus aureus by a Sensitive Immuno-PCR Assay

Su-Hua Huang1,a and Tsung-Chain Chang2

1 Division of Biological Science and Technology, MORE TOMY CO., LTD., Hsin-Chu City, Taiwan;2 Department of Medical Technology, Medical College, National Cheng Kung University, Tainan, Taiwan;

aaddress correspondence to this author at: Division of Biological Science and Technology, MORE TOMY CO., LTD., No. 194, Zing-fu St., Hsin-Chu City 300, Taiwan; fax 886-3-5339588, e-mail moretek.hcwei@msa.hinet.net

The first 20% of the full text of this article appears below.

Staphylococcus aureus causes a wide variety of diseases in humans, the clinical courses of which range from boils and furuncles to more serious diseases such as septicemia and pneumonia (1). Although a significant cause of community-acquired infection, most life-threatening cases of S. aureus disease are hospital-acquired and are associated, in many cases, with indwelling vascular devices or catheters (2). The standard method of diagnosing S. aureus is to culture an isolate from a blood agar plate and then use a latex test to identify S. aureus.

A specific product of S. aureus is protein A (3). Protein A is a cell-wall constituent of S. aureus and is mainly covalently linked to the peptidoglycan structure (4); however, ~8–30% of the protein is secreted into the medium during the exponential growth phase (5). This property has been used to develop a latex agglutination test and an ELISA to identify and detect S. aureus. We describe here a sensitive immuno-PCR assay to detect S. aureus protein A. After optimization of the reaction conditions and the use of flexible plates with 96 V-bottomed wells, which were compatible with both the ELISA washer and the thermal cycler for PCR, we automated both detection methods.

Anti-protein A antisera . . . [Full Text of this Article]






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