Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 50: 1707-1709, 2004; 10.1373/clinchem.2004.035451
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pagani, F.
Right arrow Articles by Panteghini, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pagani, F.
Right arrow Articles by Panteghini, M.
Related Collections
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2004;50:1707-1709.)
© 2004 American Association for Clinical Chemistry, Inc.

Letters to the Editor

Multicenter Evaluation of the TOSOH AIA-Pack Second-Generation Cardiac Troponin I Assay

Franca Pagani1, Francesca Stefini1, Gianmatteo Micca2, Mario Toppino2, Fabio Manoni3, Luigi Romano4, Paolo Hoffer5, Annalisa Iervasi6, Marco Caputo7, Romolo Dorizzi7, Giancarlo Zucchelli6 and Mauro Panteghini1,a

1 Laboratorio Analisi Chimico Cliniche 1 Azienda Ospedaliera ‘Spedali Civili’, Brescia, Italy
2 Laboratorio Analisi Ospedale Civile, Alba (CN), Italy
3 Patologia Clinica Ospedale Civile, Monselice (PD), Italy
4 Laboratorio Analisi Ospedale ‘Vincenzo Monaldi’, Napoli, Italy
5 Patologia Clinica Ospedale Civile, Piove di Sacco (PD), Italy
6 Laboratorio Analisi Istituto di Fisiologia Clinica Consiglio Nazionale delle Ricerche, Pisa, Italy
7 Laboratorio Analisi Ospedale Civile ‘Borgo Trento’, Verona, Italy

aAddress correspondence to this author at: Laboratorio Analisi Chimico Cliniche 1, Azienda Ospedaliera ‘Spedali Civili’, 25125 Brescia, Italy. Fax 39-030-3995369; e-mail panteghi@bshosp.osp.unibs.it.

The first 20% of the full text of this article appears below.


To the Editor:

The redefined biochemical criterion proposed to diagnose myocardial infarction (MI) necessitates the availability of highly sensitive and precise cardiac troponin assays (1). In general, a substantial improvement in the analytical performance is offered by the newer assays. In this multicenter study, we evaluated one of these improved cardiac troponin I (cTnI) assays, performed on the AIA 21 immunoassay system (TOSOH Corp.), using criteria tailored in accordance with IFCC recommendations (2).

The AIA-Pack second-generation cTnI assay uses a combination of two monoclonal antibodies, one directed to amino acids 41–49 of the cTnI molecule and another directed to amino acids 87–91. A human cardiac ternary troponin ITC complex is used as calibration antigen. The minimum detectable cTnI concentration was defined as the value corresponding to a signal 3 SD greater than the mean of 20 replicates of the zero calibrator in a single run. Buffered stock solutions of both free cTnI and troponin IC complex (Scripps Laboratories) were serially diluted in AIA-Pack diluent and tested to estimate the degree of equimolarity. Four cTnI-rich serum pools (native concentrations, ~2.5 to ~100 µg/L) were serially diluted with serum pools having cTnI below the detection limit or with the manufacturer’s diluent. The undiluted sample and four dilutions were assayed in duplicate, and the curve obtained was tested for linearity (3).

To evaluate imprecision, LiquichekTM control sera (three concentrations; Bio-Rad Laboratories) were tested in duplicate in one run per day for 20 days, using two reagent lots and two calibrations. Seven serum pools (cTnI concentrations, 0.02, 0.05, 0.10, 0.15, . . . [Full Text of this Article]




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
M. Panteghini
Selection of Antibodies and Epitopes for Cardiac Troponin Immunoassays: Should We Revise Our Evidence-Based Beliefs?
Clin. Chem., May 1, 2005; 51(5): 803 - 804.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Association for Clinical Chemistry.